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Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates

The aim of this study was to improve organic acids production in semi and continuous anaerobic treatment of Palm Oil Mill Effluent (POME) using a locally fabricated 50 L Continuously Stirred Tank Reactor (CSTR). The organic acids obtained were then used for polyhydroxyalkanoate (PHA) production by R...

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Main Authors: Hong, S. K., Shirai, Yoshihito, Abdul Rahman, Nor'aini, Hassan, Mohd Ali
Format: Article
Language:English
Published: Academic Journals 2009
Online Access:http://psasir.upm.edu.my/id/eprint/16662/1/Semi%20and%20continuous%20anaerobic%20treatment%20of%20palm%20oil%20mill%20effluent%20for%20the%20production%20of%20organic%20acids%20and%20polyhydroxyalkanoates.pdf
http://psasir.upm.edu.my/id/eprint/16662/
http://www.scialert.net/abstract/?doi=rjes.2009.552.559
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spelling my.upm.eprints.166622016-09-02T06:38:17Z http://psasir.upm.edu.my/id/eprint/16662/ Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates Hong, S. K. Shirai, Yoshihito Abdul Rahman, Nor'aini Hassan, Mohd Ali The aim of this study was to improve organic acids production in semi and continuous anaerobic treatment of Palm Oil Mill Effluent (POME) using a locally fabricated 50 L Continuously Stirred Tank Reactor (CSTR). The organic acids obtained were then used for polyhydroxyalkanoate (PHA) production by Ralstonia eutropha ATCC 17699 in a 2 L bioreactor. The conditions used in the anaerobic treatment were controlled pH 6.5, sludge to POME ratio at 1:1 and Hydraulic Retention Time (HRT) of 4 days. The organic acids obtained were about 15 g L-1 at steady state for both treatments and the organic acids yield (based on BOD) was 58.3%. The selected organic acids obtained were acetic (51.5%), propionic (27%) and butyric (21.5%) acid. The recovery of the clarified and concentrated organic acids from the treated POME was made using a two-steps evaporation process. The clarified organic acids (distillate) obtained were comprising of 44.6, 20.1 and 22.5 g L-1 acetic, propionic and butyric acids, respectively with a recovery of 76%. The organic acids collected were then used for polyhydroxyalkanoates accumulation by Ralstonia eutropha ATCC 17699 using pH stat fed-batch fermentation under nitrogen limitation of C/N 40 in a 2 L fermenter. The highest PHA concentration of 11.4 g L-1 (>90% w/w) was achieved in this process. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16662/1/Semi%20and%20continuous%20anaerobic%20treatment%20of%20palm%20oil%20mill%20effluent%20for%20the%20production%20of%20organic%20acids%20and%20polyhydroxyalkanoates.pdf Hong, S. K. and Shirai, Yoshihito and Abdul Rahman, Nor'aini and Hassan, Mohd Ali (2009) Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates. Research Journal of Environmental Sciences, 3 (5). pp. 552-559. ISSN 1819-3412; ESSN: 2152-8238 http://www.scialert.net/abstract/?doi=rjes.2009.552.559 10.3923/rjes.2009.552.559
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of this study was to improve organic acids production in semi and continuous anaerobic treatment of Palm Oil Mill Effluent (POME) using a locally fabricated 50 L Continuously Stirred Tank Reactor (CSTR). The organic acids obtained were then used for polyhydroxyalkanoate (PHA) production by Ralstonia eutropha ATCC 17699 in a 2 L bioreactor. The conditions used in the anaerobic treatment were controlled pH 6.5, sludge to POME ratio at 1:1 and Hydraulic Retention Time (HRT) of 4 days. The organic acids obtained were about 15 g L-1 at steady state for both treatments and the organic acids yield (based on BOD) was 58.3%. The selected organic acids obtained were acetic (51.5%), propionic (27%) and butyric (21.5%) acid. The recovery of the clarified and concentrated organic acids from the treated POME was made using a two-steps evaporation process. The clarified organic acids (distillate) obtained were comprising of 44.6, 20.1 and 22.5 g L-1 acetic, propionic and butyric acids, respectively with a recovery of 76%. The organic acids collected were then used for polyhydroxyalkanoates accumulation by Ralstonia eutropha ATCC 17699 using pH stat fed-batch fermentation under nitrogen limitation of C/N 40 in a 2 L fermenter. The highest PHA concentration of 11.4 g L-1 (>90% w/w) was achieved in this process.
format Article
author Hong, S. K.
Shirai, Yoshihito
Abdul Rahman, Nor'aini
Hassan, Mohd Ali
spellingShingle Hong, S. K.
Shirai, Yoshihito
Abdul Rahman, Nor'aini
Hassan, Mohd Ali
Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
author_facet Hong, S. K.
Shirai, Yoshihito
Abdul Rahman, Nor'aini
Hassan, Mohd Ali
author_sort Hong, S. K.
title Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
title_short Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
title_full Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
title_fullStr Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
title_full_unstemmed Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
title_sort semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates
publisher Academic Journals
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16662/1/Semi%20and%20continuous%20anaerobic%20treatment%20of%20palm%20oil%20mill%20effluent%20for%20the%20production%20of%20organic%20acids%20and%20polyhydroxyalkanoates.pdf
http://psasir.upm.edu.my/id/eprint/16662/
http://www.scialert.net/abstract/?doi=rjes.2009.552.559
_version_ 1643826280791015424
score 13.211869

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