Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt.
The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5%...
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2009
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my.upm.eprints.166542015-10-05T05:51:11Z http://psasir.upm.edu.my/id/eprint/16654/ Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5% and 3% (w/w) NaCl. With 3% (w/w) NaCl, the logarithmic partition coefficient of RNA was 1.2 and as a result, the RNA concentration in the top phase was 3.3-fold higher than that in the bottom phase. It is demonstrated that 47%, 13.7% and 7.5% (w/v) of PEG were required to achieve identical precipitation effects with PEG 300, 1450 and 6000, respectively. The precipitation efficiency of 6.3% (w/v) PEG 300 corresponds to that of 1% (w/v) PEG 6000. The excluded volume effects in the top phase were probably responsible for the selective exclusion of different nucleic acids species. The results obtained in this study contribute to the basic knowledge of partition of macromolecules in ATPSs in terms of excluded volume theory. Elsevier 2009-06-30 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16654/1/Selective%20partition%20of%20plasmid%20DNA%20and%20RNA%20in%20aqueous%20two.pdf Luechau, Frank and Ling, Tau Chuan and Lyddiatt, Andrew (2009) Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. Separation and Purification Technology, 68 (1). pp. 114-118. ISSN 1383-5866 10.1016/j.seppur.2009.04.016 English |
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The selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPSs) by addition of NaCl salt was studied with pure pDNA and RNA solutions. The pDNA is increasingly excluded from top phases upon the addition of 0.5% and 3% (w/w) NaCl. With 3% (w/w) NaCl, the logarithmic partition coefficient of RNA was 1.2 and as a result, the RNA concentration in the top phase was 3.3-fold higher than that in the bottom phase. It is demonstrated that 47%, 13.7% and 7.5% (w/v) of PEG were required to achieve identical precipitation effects with PEG 300, 1450 and 6000, respectively. The precipitation efficiency of 6.3% (w/v) PEG 300 corresponds to that of 1% (w/v) PEG 6000. The excluded volume effects in the top phase were probably responsible for the selective exclusion of different nucleic acids species. The results obtained in this study contribute to the basic knowledge of partition of macromolecules in ATPSs in terms of excluded volume theory. |
| format |
Article |
| author |
Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew |
| spellingShingle |
Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. |
| author_facet |
Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew |
| author_sort |
Luechau, Frank |
| title |
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. |
| title_short |
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. |
| title_full |
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. |
| title_fullStr |
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. |
| title_full_unstemmed |
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt. |
| title_sort |
selective partition of plasmid dna and rna in aqueous two-phase systems by the addition of neutral salt. |
| publisher |
Elsevier |
| publishDate |
2009 |
| url |
http://psasir.upm.edu.my/id/eprint/16654/1/Selective%20partition%20of%20plasmid%20DNA%20and%20RNA%20in%20aqueous%20two.pdf http://psasir.upm.edu.my/id/eprint/16654/ |
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1643826278427525120 |
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13.211869 |