Cover Image

Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells

Phytic acid (inositol hexaphosphate or IP6) is one of the bioactive compound that is present in cereals, nuts and legumes. IP6 is a naturally occurring polyphosphorylated carbohydrate, recognized to posses various significant health benefits including anticancer effects. Several in vitro and in vivo...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohd Esa, Norhaizan, Md. Akim, Abdah, Ithnin, Hairuszah, Shafie, Nurul-Husna, Saad, Norazalina, Ismail, Norsharina
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/16578/1/Rice%20bran%20phytic%20acid.pdf
http://psasir.upm.edu.my/id/eprint/16578/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.16578
record_format eprints
spelling my.upm.eprints.165782016-06-30T03:20:27Z http://psasir.upm.edu.my/id/eprint/16578/ Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells Mohd Esa, Norhaizan Md. Akim, Abdah Ithnin, Hairuszah Shafie, Nurul-Husna Saad, Norazalina Ismail, Norsharina Phytic acid (inositol hexaphosphate or IP6) is one of the bioactive compound that is present in cereals, nuts and legumes. IP6 is a naturally occurring polyphosphorylated carbohydrate, recognized to posses various significant health benefits including anticancer effects. Several in vitro and in vivo studies provide convincing evidence for the anticarcinogenic properties of commercial rice IP6 whilst the underlying mechanisms by which IP6 exerts anti-tumorigenic effects are still not fully known. The purpose of this present study is to investigate the growth inhibitory effects of IP6 extracted from rice bran on human colorectal cancer cell line (HT-29). IP6 extracted from rice bran induced marked growth inhibition in HT-29 with an IC50 value of 12.0 ± 2 μg/ml, in a dose and time dependent manner. Flow cytometry was performed for the analysis of cell cycle and apoptosis. Rice bran IP6-extract induced cell cycle arrest in HT-29 cell at G0/G1 phase. Staining with Annexin V-based assay and propidium iodide confirmed that apoptosis occurred early and late in the HT-29. IP6 is expected to exert anticarcinogenic activity through disruption of cell cycle progression and induction of apoptosis. Our study further supports the function of rice bran IP6 as a chemopreventive agent for human colorectal cancer. Academic Journals 2010-11-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16578/1/Rice%20bran%20phytic%20acid.pdf Mohd Esa, Norhaizan and Md. Akim, Abdah and Ithnin, Hairuszah and Shafie, Nurul-Husna and Saad, Norazalina and Ismail, Norsharina (2010) Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells. Journal of Medicinal Plants Research, 4 (21). pp. 2283-2289. ISSN 1996-0875 10.5897/JMPR10.519
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Phytic acid (inositol hexaphosphate or IP6) is one of the bioactive compound that is present in cereals, nuts and legumes. IP6 is a naturally occurring polyphosphorylated carbohydrate, recognized to posses various significant health benefits including anticancer effects. Several in vitro and in vivo studies provide convincing evidence for the anticarcinogenic properties of commercial rice IP6 whilst the underlying mechanisms by which IP6 exerts anti-tumorigenic effects are still not fully known. The purpose of this present study is to investigate the growth inhibitory effects of IP6 extracted from rice bran on human colorectal cancer cell line (HT-29). IP6 extracted from rice bran induced marked growth inhibition in HT-29 with an IC50 value of 12.0 ± 2 μg/ml, in a dose and time dependent manner. Flow cytometry was performed for the analysis of cell cycle and apoptosis. Rice bran IP6-extract induced cell cycle arrest in HT-29 cell at G0/G1 phase. Staining with Annexin V-based assay and propidium iodide confirmed that apoptosis occurred early and late in the HT-29. IP6 is expected to exert anticarcinogenic activity through disruption of cell cycle progression and induction of apoptosis. Our study further supports the function of rice bran IP6 as a chemopreventive agent for human colorectal cancer.
format Article
author Mohd Esa, Norhaizan
Md. Akim, Abdah
Ithnin, Hairuszah
Shafie, Nurul-Husna
Saad, Norazalina
Ismail, Norsharina
spellingShingle Mohd Esa, Norhaizan
Md. Akim, Abdah
Ithnin, Hairuszah
Shafie, Nurul-Husna
Saad, Norazalina
Ismail, Norsharina
Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
author_facet Mohd Esa, Norhaizan
Md. Akim, Abdah
Ithnin, Hairuszah
Shafie, Nurul-Husna
Saad, Norazalina
Ismail, Norsharina
author_sort Mohd Esa, Norhaizan
title Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
title_short Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
title_full Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
title_fullStr Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
title_full_unstemmed Rice bran phytic acid (IP6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
title_sort rice bran phytic acid (ip6) induces growth inhibition, cell cycle arrest and apoptosis on human colorectal adenocarcinoma cells
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/16578/1/Rice%20bran%20phytic%20acid.pdf
http://psasir.upm.edu.my/id/eprint/16578/
_version_ 1643826255030648832
score 13.211869

Similar Items