Cover Image

Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia

The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantificatio...

Full description

Saved in:
Bibliographic Details
Main Authors: Kesar Singh, Jasbeer Kaur, Radu, Son, Mohamad Ghazali, Farinazleen, Cheah, Yoke Kqueen
Format: Article
Language:English
Published: Elsevier 2010
Online Access:http://psasir.upm.edu.my/id/eprint/16440/1/Real.pdf
http://psasir.upm.edu.my/id/eprint/16440/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.16440
record_format eprints
spelling my.upm.eprints.164402015-09-27T23:40:06Z http://psasir.upm.edu.my/id/eprint/16440/ Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia Kesar Singh, Jasbeer Kaur Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia. Elsevier 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16440/1/Real.pdf Kesar Singh, Jasbeer Kaur and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen (2010) Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia. Food Control, 21 (11). pp. 1536-1544. ISSN 0956-7135; ESSN: 1873-7129 10.1016/j.foodcont.2010.03.018
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia.
format Article
author Kesar Singh, Jasbeer Kaur
Radu, Son
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
spellingShingle Kesar Singh, Jasbeer Kaur
Radu, Son
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
author_facet Kesar Singh, Jasbeer Kaur
Radu, Son
Mohamad Ghazali, Farinazleen
Cheah, Yoke Kqueen
author_sort Kesar Singh, Jasbeer Kaur
title Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
title_short Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
title_full Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
title_fullStr Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
title_full_unstemmed Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
title_sort real-time pcr-based detection and quantification of genetically modified maize in processed feeds commercialised in malaysia
publisher Elsevier
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/16440/1/Real.pdf
http://psasir.upm.edu.my/id/eprint/16440/
_version_ 1643826215028523008
score 13.211869

Similar Items