Staff View: Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems

Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems

Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium ph...

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Main Authors:	Ooi, Chien Wei, Tey, Beng Ti, Hii, Siew Ling, Mustapa Kamal, Siti Mazlina, Chi, John Wei Lan, Ariff, Arbakariya, Tau, Chuan Ling
Format:	Article
Published:	Elsevier 2009
Online Access:	http://psasir.upm.edu.my/id/eprint/16396/ http://www.sciencedirect.com/science/article/pii/S1359511309001718
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id	my.upm.eprints.16396
record_format	eprints
spelling	my.upm.eprints.163962015-09-25T02:25:59Z http://psasir.upm.edu.my/id/eprint/16396/ Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%. Elsevier 2009-10 Article PeerReviewed Ooi, Chien Wei and Tey, Beng Ti and Hii, Siew Ling and Mustapa Kamal, Siti Mazlina and Chi, John Wei Lan and Ariff, Arbakariya and Tau, Chuan Ling (2009) Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems. Process Biochemistry, 44 (10). pp. 1083-1087. ISSN 1359-5113; ESSN: 1873-3298 http://www.sciencedirect.com/science/article/pii/S1359511309001718 10.1016/j.procbio.2009.05.008
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%.
format	Article
author	Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling
spellingShingle	Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
author_facet	Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling
author_sort	Ooi, Chien Wei
title	Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
title_short	Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
title_full	Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
title_fullStr	Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
title_full_unstemmed	Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
title_sort	purification of lipase derived from burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
publisher	Elsevier
publishDate	2009
url	http://psasir.upm.edu.my/id/eprint/16396/ http://www.sciencedirect.com/science/article/pii/S1359511309001718
_version_	1643826201511329792
score	13.211869

Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems

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