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Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria

An experiment was conducted to determine the production of hydrolytic enzymes endoglucanase (EG) and endopolymethylgalacturonase (EPMG) in rice (Oryza sativa L.) roots inoculated with N2-Fixing bacteria. Screening for hydrolytic enzymes by N2-Fixing bacteria, using the plate method showed that ni...

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Main Authors: A. M, Asilah, O, Radziah, M, Radzali
Format: Article
Language:English
English
Published: Malaysian Society of Soil Science 2009
Online Access:http://psasir.upm.edu.my/id/eprint/16359/1/Production%20of%20hydrolytic%20enzymes%20in%20rice.pdf
http://psasir.upm.edu.my/id/eprint/16359/
http://www.msss.com.my/mjss/Full%20Text/Vol%2013/asilah.pdf
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spelling my.upm.eprints.163592015-11-26T07:04:23Z http://psasir.upm.edu.my/id/eprint/16359/ Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria A. M, Asilah O, Radziah M, Radzali An experiment was conducted to determine the production of hydrolytic enzymes endoglucanase (EG) and endopolymethylgalacturonase (EPMG) in rice (Oryza sativa L.) roots inoculated with N2-Fixing bacteria. Screening for hydrolytic enzymes by N2-Fixing bacteria, using the plate method showed that nine out of 12 bacterial strains were positive for carboxymethylcellulose (CMC) and pectin reactions. Three of the isolates, Sb34, Sb41 and Sb42 were inoculated to MR219 rice seedling. The bacterial population and the production of hydrolytic enzymes were monitored for 45 days of plant growth. The scanning (SEM) and transmission electron microscopy (TEM) were used to observe bacterial colonization on plant roots. In general, the populations of inoculated diazotrophs were higher in the rhizosphere than the endosphere. There were significant effects of different diazotrophs inoculations on the rice rhizosphere and endosphere populations. Plants inoculated with diazotrophs showed significantly higher specific enzyme activities and soluble proteins compared to the non-inoculated control. SEM and TEM observations revealed the abilities of the diazotrophs to colonize the surfaces and interior of the roots. Inoculation significantly increased root growth of rice with substantial increase in root length, volume and surface area in the inoculated plants. Malaysian Society of Soil Science 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16359/1/Production%20of%20hydrolytic%20enzymes%20in%20rice.pdf A. M, Asilah and O, Radziah and M, Radzali (2009) Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria. Malaysian Journal of Soil Science, 13. pp. 43-57. ISSN 1394-7990 http://www.msss.com.my/mjss/Full%20Text/Vol%2013/asilah.pdf English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description An experiment was conducted to determine the production of hydrolytic enzymes endoglucanase (EG) and endopolymethylgalacturonase (EPMG) in rice (Oryza sativa L.) roots inoculated with N2-Fixing bacteria. Screening for hydrolytic enzymes by N2-Fixing bacteria, using the plate method showed that nine out of 12 bacterial strains were positive for carboxymethylcellulose (CMC) and pectin reactions. Three of the isolates, Sb34, Sb41 and Sb42 were inoculated to MR219 rice seedling. The bacterial population and the production of hydrolytic enzymes were monitored for 45 days of plant growth. The scanning (SEM) and transmission electron microscopy (TEM) were used to observe bacterial colonization on plant roots. In general, the populations of inoculated diazotrophs were higher in the rhizosphere than the endosphere. There were significant effects of different diazotrophs inoculations on the rice rhizosphere and endosphere populations. Plants inoculated with diazotrophs showed significantly higher specific enzyme activities and soluble proteins compared to the non-inoculated control. SEM and TEM observations revealed the abilities of the diazotrophs to colonize the surfaces and interior of the roots. Inoculation significantly increased root growth of rice with substantial increase in root length, volume and surface area in the inoculated plants.
format Article
author A. M, Asilah
O, Radziah
M, Radzali
spellingShingle A. M, Asilah
O, Radziah
M, Radzali
Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria
author_facet A. M, Asilah
O, Radziah
M, Radzali
author_sort A. M, Asilah
title Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria
title_short Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria
title_full Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria
title_fullStr Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria
title_full_unstemmed Production of hydrolytic enzymes in rice (Oryza sativa L.) roots inoculated with N2-fixing bacteria
title_sort production of hydrolytic enzymes in rice (oryza sativa l.) roots inoculated with n2-fixing bacteria
publisher Malaysian Society of Soil Science
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16359/1/Production%20of%20hydrolytic%20enzymes%20in%20rice.pdf
http://psasir.upm.edu.my/id/eprint/16359/
http://www.msss.com.my/mjss/Full%20Text/Vol%2013/asilah.pdf
_version_ 1643826190793834496
score 13.211869

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