Cover Image

Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level

The purpose of this study was to investigate the biosafety of Vibrio parahaemolyticus in raw salad vegetables at wet markets and supermarkets in Malaysia. A combination of the most probable number -polymerase chain reaction (MPN- PCR) method was applied to detect the presence of V. parahaemoly...

Full description

Saved in:
Bibliographic Details
Main Authors: Robin, Tunung, Paie, Margaret Selina, Ponniah, Jeyaletchumi, Chai, Lay Ching, Tuan Chilek, Tuan Zainazor, Mohamad Ghazali, Farinazleen, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Radu, Son
Format: Article
Language:English
English
Published: The Korean Society for Applied Microbiology 2010
Online Access:http://psasir.upm.edu.my/id/eprint/16326/1/Prevalence%20and%20quantification%20of%20Vibrio%20parahaemolyticus%20in%20raw%20salad%20vegetables%20at%20retail%20level.pdf
http://psasir.upm.edu.my/id/eprint/16326/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.16326
record_format eprints
spelling my.upm.eprints.163262015-11-02T06:39:42Z http://psasir.upm.edu.my/id/eprint/16326/ Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level Robin, Tunung Paie, Margaret Selina Ponniah, Jeyaletchumi Chai, Lay Ching Tuan Chilek, Tuan Zainazor Mohamad Ghazali, Farinazleen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son The purpose of this study was to investigate the biosafety of Vibrio parahaemolyticus in raw salad vegetables at wet markets and supermarkets in Malaysia. A combination of the most probable number -polymerase chain reaction (MPN- PCR) method was applied to detect the presence of V. parahaemolyticus and to enumerate their density in the food samples. The study analyzed 276 samples of common vegetables eaten raw in Malaysia (Wild cosmos=8; Japanese parsley=21; Cabbage=30; Lettuce=16; Indian pennywort=17; Carrot=31; Sweet potato=29; Tomato=38; Cucumber=28; Four-winged bean=26; Long bean=32). The samples were purchased from two supermarkets (A and B) and two wet markets (C and D). The occurrence of V. parahaemolyticus detected was 20.65%, with a higher frequency of V. parahaemolyticus in vegetables obtained from wet markets (Wet market C=27.27%; Wet Market D=32.05%) compared with supermarkets (Supermarket A=1.64%; Supermarket B=16.67%). V. parahaemolyticus was most prevalent in Indian pennywort (41.18%). The density of V. parahaemolyticus in all the samples ranged from <3 up to >2,400 MPN/g, mostly <3 MPN/g concentration. Raw vegetables from wet markets contained higher levels of V. parahaemolyticus compared with supermarkets. Although V. parahaemolyticus was present in raw vegetables, its numbers were low. The results suggest that raw vegetables act as a transmission route for V. parahaemolyticus. This study will be the first biosafety assessment of V. parahaemolyticus in raw vegetables in Malaysia. The Korean Society for Applied Microbiology 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16326/1/Prevalence%20and%20quantification%20of%20Vibrio%20parahaemolyticus%20in%20raw%20salad%20vegetables%20at%20retail%20level.pdf Robin, Tunung and Paie, Margaret Selina and Ponniah, Jeyaletchumi and Chai, Lay Ching and Tuan Chilek, Tuan Zainazor and Mohamad Ghazali, Farinazleen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2010) Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level. Journal of Microbiology and Biotechnology, 20 (2). pp. 391-396. ISSN 1017-7825 10.4014/jmb.0908.08009 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The purpose of this study was to investigate the biosafety of Vibrio parahaemolyticus in raw salad vegetables at wet markets and supermarkets in Malaysia. A combination of the most probable number -polymerase chain reaction (MPN- PCR) method was applied to detect the presence of V. parahaemolyticus and to enumerate their density in the food samples. The study analyzed 276 samples of common vegetables eaten raw in Malaysia (Wild cosmos=8; Japanese parsley=21; Cabbage=30; Lettuce=16; Indian pennywort=17; Carrot=31; Sweet potato=29; Tomato=38; Cucumber=28; Four-winged bean=26; Long bean=32). The samples were purchased from two supermarkets (A and B) and two wet markets (C and D). The occurrence of V. parahaemolyticus detected was 20.65%, with a higher frequency of V. parahaemolyticus in vegetables obtained from wet markets (Wet market C=27.27%; Wet Market D=32.05%) compared with supermarkets (Supermarket A=1.64%; Supermarket B=16.67%). V. parahaemolyticus was most prevalent in Indian pennywort (41.18%). The density of V. parahaemolyticus in all the samples ranged from <3 up to >2,400 MPN/g, mostly <3 MPN/g concentration. Raw vegetables from wet markets contained higher levels of V. parahaemolyticus compared with supermarkets. Although V. parahaemolyticus was present in raw vegetables, its numbers were low. The results suggest that raw vegetables act as a transmission route for V. parahaemolyticus. This study will be the first biosafety assessment of V. parahaemolyticus in raw vegetables in Malaysia.
format Article
author Robin, Tunung
Paie, Margaret Selina
Ponniah, Jeyaletchumi
Chai, Lay Ching
Tuan Chilek, Tuan Zainazor
Mohamad Ghazali, Farinazleen
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
spellingShingle Robin, Tunung
Paie, Margaret Selina
Ponniah, Jeyaletchumi
Chai, Lay Ching
Tuan Chilek, Tuan Zainazor
Mohamad Ghazali, Farinazleen
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
author_facet Robin, Tunung
Paie, Margaret Selina
Ponniah, Jeyaletchumi
Chai, Lay Ching
Tuan Chilek, Tuan Zainazor
Mohamad Ghazali, Farinazleen
Nakaguchi, Yoshitsugu
Nishibuchi, Mitsuaki
Radu, Son
author_sort Robin, Tunung
title Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
title_short Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
title_full Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
title_fullStr Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
title_full_unstemmed Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
title_sort prevalence and quantification of vibrio parahaemolyticus in raw salad vegetables at retail level
publisher The Korean Society for Applied Microbiology
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/16326/1/Prevalence%20and%20quantification%20of%20Vibrio%20parahaemolyticus%20in%20raw%20salad%20vegetables%20at%20retail%20level.pdf
http://psasir.upm.edu.my/id/eprint/16326/
_version_ 1643826180972871680
score 13.211869

Similar Items