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Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak

Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing ba...

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Main Authors: Sabullah, Mohd Khalizan, Rahman, M. F., Ahmad, Siti Aqlima, Sulaiman, Mohd Rosni, Shukor, Mohd Shukri, Shamaan, Nor Aripin, Shukor, Mohd Yunus
Format: Article
Language:English
Published: Faculty of Agriculture, University of Brawijaya, Indonesia 2016
Online Access:http://psasir.upm.edu.my/id/eprint/16206/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20Saw-5%20in%20soils%20from%20Sarawak.pdf
http://psasir.upm.edu.my/id/eprint/16206/
http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/654
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spelling my.upm.eprints.162062016-06-08T01:15:22Z http://psasir.upm.edu.my/id/eprint/16206/ Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak Sabullah, Mohd Khalizan Rahman, M. F. Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Shukor, Mohd Yunus Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. Faculty of Agriculture, University of Brawijaya, Indonesia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16206/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20Saw-5%20in%20soils%20from%20Sarawak.pdf Sabullah, Mohd Khalizan and Rahman, M. F. and Ahmad, Siti Aqlima and Sulaiman, Mohd Rosni and Shukor, Mohd Shukri and Shamaan, Nor Aripin and Shukor, Mohd Yunus (2016) Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak. Agrivita, 38 (1). pp. 1-13. ISSN 0126-0537; ESSN: 2477-8516 http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/654
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.
format Article
author Sabullah, Mohd Khalizan
Rahman, M. F.
Ahmad, Siti Aqlima
Sulaiman, Mohd Rosni
Shukor, Mohd Shukri
Shamaan, Nor Aripin
Shukor, Mohd Yunus
spellingShingle Sabullah, Mohd Khalizan
Rahman, M. F.
Ahmad, Siti Aqlima
Sulaiman, Mohd Rosni
Shukor, Mohd Shukri
Shamaan, Nor Aripin
Shukor, Mohd Yunus
Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
author_facet Sabullah, Mohd Khalizan
Rahman, M. F.
Ahmad, Siti Aqlima
Sulaiman, Mohd Rosni
Shukor, Mohd Shukri
Shamaan, Nor Aripin
Shukor, Mohd Yunus
author_sort Sabullah, Mohd Khalizan
title Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
title_short Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
title_full Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
title_fullStr Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
title_full_unstemmed Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
title_sort isolation and characterization of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in soils from sarawak
publisher Faculty of Agriculture, University of Brawijaya, Indonesia
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/16206/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20Saw-5%20in%20soils%20from%20Sarawak.pdf
http://psasir.upm.edu.my/id/eprint/16206/
http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/654
_version_ 1643826144379666432
score 13.211869

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