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p16 gene expression in basal cell carcinoma.

Background: Basal cell carcinoma (BCC) develops predominantly in sun-exposed skin in fair-skinned individuals prone to sunburn. BCC typically occurs in adults. High exposure to ultraviolet (UV) radiation increases rate of developing BCC, a slowly growing tumor that occurs in hair-growing squamous...

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Main Authors: Eshkoor, Sima Atoallahi, Ismail, Patimah, Abdul Rahman, Sabariah, Oshkour, Soraya Ataollahi
Format: Article
Language:English
Published: Elsevier 2008
Online Access:http://psasir.upm.edu.my/id/eprint/16020/1/p16%20gene%20expression%20in%20basal%20cell%20carcinoma.pdf
http://psasir.upm.edu.my/id/eprint/16020/
http://www.elsevier.com/
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spelling my.upm.eprints.160202015-09-14T08:00:22Z http://psasir.upm.edu.my/id/eprint/16020/ p16 gene expression in basal cell carcinoma. Eshkoor, Sima Atoallahi Ismail, Patimah Abdul Rahman, Sabariah Oshkour, Soraya Ataollahi Background: Basal cell carcinoma (BCC) develops predominantly in sun-exposed skin in fair-skinned individuals prone to sunburn. BCC typically occurs in adults. High exposure to ultraviolet (UV) radiation increases rate of developing BCC, a slowly growing tumor that occurs in hair-growing squamous epithelium and rarely metastasizes. In genetic studies, BCC patients have cell-cycle abnormalities of different parts of the signaling pathway. Retinoblastoma regulatory pathway is important in cell cycle arrest. In this pathway, p16INK4a, an inhibitor of Rb pathway, binds to CDK4 and CDK6 competitively with cyclin D1 to prevent phosphorylation of tumor suppressor pRB gene. Alteration of this pathway contributes to development of human cancers and also is effective in skin cancers. In this study, we analyzed mRNA expression using in situ RT-PCR and the role of immunohistochemical expression of p16INK4a in BCC. Methods: Expression of p16 in ten samples of Iranian paraffin-embedded skin BCC were studied using in situ RT-PCR and immunohistochemistry on p16INK4a gene. Results: Nuclear and cytoplasmic staining intensity of samples within tumor cells and normal skin tissue illustrates different mRNA and protein expression of p16 gene. mRNA of p16 gene and the expressed protein induce cell cycle proliferation and involve both tumor tissue as well as normal skin tissue. However, in this study it was found that there is significant protein and mRNA expression in BCC cells when compared to normal skin tissue (p <0.05). Conclusions: p16 gene is involved in the pathogenesis of human skin BCC in view of increased p16 mRNA and expressed protein within tumor cells. Elsevier 2008-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16020/1/p16%20gene%20expression%20in%20basal%20cell%20carcinoma.pdf Eshkoor, Sima Atoallahi and Ismail, Patimah and Abdul Rahman, Sabariah and Oshkour, Soraya Ataollahi (2008) p16 gene expression in basal cell carcinoma. Archives of Medical Research, 39 (7). pp. 668-673. ISSN 0188-4409, ESSN: 1873-5487 http://www.elsevier.com/ 10.1016/j.arcmed.2008.06.003
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Basal cell carcinoma (BCC) develops predominantly in sun-exposed skin in fair-skinned individuals prone to sunburn. BCC typically occurs in adults. High exposure to ultraviolet (UV) radiation increases rate of developing BCC, a slowly growing tumor that occurs in hair-growing squamous epithelium and rarely metastasizes. In genetic studies, BCC patients have cell-cycle abnormalities of different parts of the signaling pathway. Retinoblastoma regulatory pathway is important in cell cycle arrest. In this pathway, p16INK4a, an inhibitor of Rb pathway, binds to CDK4 and CDK6 competitively with cyclin D1 to prevent phosphorylation of tumor suppressor pRB gene. Alteration of this pathway contributes to development of human cancers and also is effective in skin cancers. In this study, we analyzed mRNA expression using in situ RT-PCR and the role of immunohistochemical expression of p16INK4a in BCC. Methods: Expression of p16 in ten samples of Iranian paraffin-embedded skin BCC were studied using in situ RT-PCR and immunohistochemistry on p16INK4a gene. Results: Nuclear and cytoplasmic staining intensity of samples within tumor cells and normal skin tissue illustrates different mRNA and protein expression of p16 gene. mRNA of p16 gene and the expressed protein induce cell cycle proliferation and involve both tumor tissue as well as normal skin tissue. However, in this study it was found that there is significant protein and mRNA expression in BCC cells when compared to normal skin tissue (p <0.05). Conclusions: p16 gene is involved in the pathogenesis of human skin BCC in view of increased p16 mRNA and expressed protein within tumor cells.
format Article
author Eshkoor, Sima Atoallahi
Ismail, Patimah
Abdul Rahman, Sabariah
Oshkour, Soraya Ataollahi
spellingShingle Eshkoor, Sima Atoallahi
Ismail, Patimah
Abdul Rahman, Sabariah
Oshkour, Soraya Ataollahi
p16 gene expression in basal cell carcinoma.
author_facet Eshkoor, Sima Atoallahi
Ismail, Patimah
Abdul Rahman, Sabariah
Oshkour, Soraya Ataollahi
author_sort Eshkoor, Sima Atoallahi
title p16 gene expression in basal cell carcinoma.
title_short p16 gene expression in basal cell carcinoma.
title_full p16 gene expression in basal cell carcinoma.
title_fullStr p16 gene expression in basal cell carcinoma.
title_full_unstemmed p16 gene expression in basal cell carcinoma.
title_sort p16 gene expression in basal cell carcinoma.
publisher Elsevier
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/16020/1/p16%20gene%20expression%20in%20basal%20cell%20carcinoma.pdf
http://psasir.upm.edu.my/id/eprint/16020/
http://www.elsevier.com/
_version_ 1643826095742517248
score 13.211869

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