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Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method

Enzymatic synthesis of xylitol fatty acid ester was performed in hexane using Novozym 435 (immobilized Candida antarctica lipase on macroporous resin). Taguchi method based on three levels, six variables L27 orthogonal array robust design was implemented to optimize experimental conditions. The effe...

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Main Authors: Adnani, Atena, Basri, Mahiran, Abd. Malek, Emilia, Salleh, Abu Bakar, Abdul Rahman, Mohd Basyaruddin, Chaibakhsh, Naz, Raja Abdul Rahman, Raja Noor Zaliha
Format: Article
Language:English
Published: Elsevier 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15975/1/Optimization%20of%20lipase.pdf
http://psasir.upm.edu.my/id/eprint/15975/
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spelling my.upm.eprints.159752016-09-02T04:31:37Z http://psasir.upm.edu.my/id/eprint/15975/ Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method Adnani, Atena Basri, Mahiran Abd. Malek, Emilia Salleh, Abu Bakar Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Raja Abdul Rahman, Raja Noor Zaliha Enzymatic synthesis of xylitol fatty acid ester was performed in hexane using Novozym 435 (immobilized Candida antarctica lipase on macroporous resin). Taguchi method based on three levels, six variables L27 orthogonal array robust design was implemented to optimize experimental conditions. The effects of reaction parameters including reaction time (7–24 h), enzyme amount (0.05–0.3 g), temperature (30–60 °C), amount of molecular sieve (1–4 g), substrate molar ratio (0.3–1) and xylitol concentration (0.005–0.015 g/ml) on the percentage yield of sugar ester were investigated. The optimum conditions derived via Taguchi method were: reaction time 7 h, temperature 60 °C, amount of enzyme 0.12 g, amount of molecular sieve 2.5 g, substrate molar ratio 1 and xylitol concentration 0.015 g/ml. The actual experimental yield was 96.10% under optimum condition, which compared well to the maximum predicted value of 96.27%. Elsevier 2010-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15975/1/Optimization%20of%20lipase.pdf Adnani, Atena and Basri, Mahiran and Abd. Malek, Emilia and Salleh, Abu Bakar and Abdul Rahman, Mohd Basyaruddin and Chaibakhsh, Naz and Raja Abdul Rahman, Raja Noor Zaliha (2010) Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method. Industrial Crops and Products, 31 (2). pp. 350-356. ISSN 0926-6690; ESSN: 1872-633X 10.1016/j.indcrop.2009.12.001
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Enzymatic synthesis of xylitol fatty acid ester was performed in hexane using Novozym 435 (immobilized Candida antarctica lipase on macroporous resin). Taguchi method based on three levels, six variables L27 orthogonal array robust design was implemented to optimize experimental conditions. The effects of reaction parameters including reaction time (7–24 h), enzyme amount (0.05–0.3 g), temperature (30–60 °C), amount of molecular sieve (1–4 g), substrate molar ratio (0.3–1) and xylitol concentration (0.005–0.015 g/ml) on the percentage yield of sugar ester were investigated. The optimum conditions derived via Taguchi method were: reaction time 7 h, temperature 60 °C, amount of enzyme 0.12 g, amount of molecular sieve 2.5 g, substrate molar ratio 1 and xylitol concentration 0.015 g/ml. The actual experimental yield was 96.10% under optimum condition, which compared well to the maximum predicted value of 96.27%.
format Article
author Adnani, Atena
Basri, Mahiran
Abd. Malek, Emilia
Salleh, Abu Bakar
Abdul Rahman, Mohd Basyaruddin
Chaibakhsh, Naz
Raja Abdul Rahman, Raja Noor Zaliha
spellingShingle Adnani, Atena
Basri, Mahiran
Abd. Malek, Emilia
Salleh, Abu Bakar
Abdul Rahman, Mohd Basyaruddin
Chaibakhsh, Naz
Raja Abdul Rahman, Raja Noor Zaliha
Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
author_facet Adnani, Atena
Basri, Mahiran
Abd. Malek, Emilia
Salleh, Abu Bakar
Abdul Rahman, Mohd Basyaruddin
Chaibakhsh, Naz
Raja Abdul Rahman, Raja Noor Zaliha
author_sort Adnani, Atena
title Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
title_short Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
title_full Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
title_fullStr Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
title_full_unstemmed Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
title_sort optimization of lipase-catalyzed synthesis of xylitol ester by taguchi robust design method
publisher Elsevier
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/15975/1/Optimization%20of%20lipase.pdf
http://psasir.upm.edu.my/id/eprint/15975/
_version_ 1643826083292774400
score 13.211869

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