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Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars

The effect of enzyme dosage and initial substrate concentration on the performance of enzymatic saccharification of palm oil mill effluent (POME) solid and oil palm fruit fibre (OPFF) was carried out in reactions using shake flask and 2-litre stirred tank reactor. The highest production of total red...

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Main Authors: Khaw, Teik Seong, Ariff, Arbakariya
Format: Article
Language:English
Published: Malaysian Agricultural Research and Development Institute (MARDI) 2009
Online Access:http://psasir.upm.edu.my/id/eprint/15969/1/15969.pdf
http://psasir.upm.edu.my/id/eprint/15969/
http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/35-2009/volume-37-no1/80-370111
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spelling my.upm.eprints.159692016-10-10T04:42:43Z http://psasir.upm.edu.my/id/eprint/15969/ Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars Khaw, Teik Seong Ariff, Arbakariya The effect of enzyme dosage and initial substrate concentration on the performance of enzymatic saccharification of palm oil mill effluent (POME) solid and oil palm fruit fibre (OPFF) was carried out in reactions using shake flask and 2-litre stirred tank reactor. The highest production of total reducing sugar and glucose from saccharification of POME and OPFF obtained using Novozyme/Celluclast (N/C ratio) were 0.4 and 0.25 at a dosage of 1 ml/g and 2 ml/g substrate, respectively. At this optimal condition, the highest production of reducing sugar (12.25 g/litre) and glucose (6.70 g/litre) was obtained when 150 g/litre POME solid was used, which gave the overall productivity and yield of 1.53 g/litre/h and 0.08 g/g, respectively. On the other hand, the saccharification of OPFF was optimal at 50 g/litre which produced 30.26 g/litre reducing sugar and 16.73 g/litre glucose, which corresponding to overall productivity of 0.28 g/litre/h and yield of 0.61 g/g. Malaysian Agricultural Research and Development Institute (MARDI) 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15969/1/15969.pdf Khaw, Teik Seong and Ariff, Arbakariya (2009) Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars. Journal of Tropical Agriculture and Food Science, 37 (1). pp. 85-94. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/35-2009/volume-37-no1/80-370111
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effect of enzyme dosage and initial substrate concentration on the performance of enzymatic saccharification of palm oil mill effluent (POME) solid and oil palm fruit fibre (OPFF) was carried out in reactions using shake flask and 2-litre stirred tank reactor. The highest production of total reducing sugar and glucose from saccharification of POME and OPFF obtained using Novozyme/Celluclast (N/C ratio) were 0.4 and 0.25 at a dosage of 1 ml/g and 2 ml/g substrate, respectively. At this optimal condition, the highest production of reducing sugar (12.25 g/litre) and glucose (6.70 g/litre) was obtained when 150 g/litre POME solid was used, which gave the overall productivity and yield of 1.53 g/litre/h and 0.08 g/g, respectively. On the other hand, the saccharification of OPFF was optimal at 50 g/litre which produced 30.26 g/litre reducing sugar and 16.73 g/litre glucose, which corresponding to overall productivity of 0.28 g/litre/h and yield of 0.61 g/g.
format Article
author Khaw, Teik Seong
Ariff, Arbakariya
spellingShingle Khaw, Teik Seong
Ariff, Arbakariya
Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
author_facet Khaw, Teik Seong
Ariff, Arbakariya
author_sort Khaw, Teik Seong
title Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
title_short Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
title_full Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
title_fullStr Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
title_full_unstemmed Optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
title_sort optimization of enzymatic saccharification of palm mill effluent solid and oil palm fruit fibre to fermentable sugars
publisher Malaysian Agricultural Research and Development Institute (MARDI)
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/15969/1/15969.pdf
http://psasir.upm.edu.my/id/eprint/15969/
http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/35-2009/volume-37-no1/80-370111
_version_ 1643826081517535232
score 13.211869

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