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Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.

Lipopolysaccharide (LPS) of Brucella abortus is an essential component for developing the subunit vaccine against brucellosis. B. abortus LPS was extracted by n-butanol, purified by ultracentrifugation and detoxified by alkaline treatment. Pyrogenicity and toxicity of B. abortus LPS and detoxified–L...

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Main Authors: Pakzad, Iraj, Rezaee, Abbas, Rasaee, Mohammad Javad, Hosseini, Ahmad Zavaran, Tabbaraee, Bahman, Ghafurian, Sobhan, Abdulamir, Ahmed Sahib, Abu Bakar, Fatimah, Mohammad, Raftari
Format: Article
Language:English
English
Published: Asian Network for Scientific Information (ANSINET) 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15842/1/Novel%20approach%20of%20vaccination%20against%20Brucella%20abortus%20544%20based%20on%20a%20combination%20of%20fusion%20proteins.pdf
http://psasir.upm.edu.my/id/eprint/15842/
http://scialert.net/abstract/?doi=jbs.2010.767.772
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spelling my.upm.eprints.158422015-10-29T03:59:06Z http://psasir.upm.edu.my/id/eprint/15842/ Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides. Pakzad, Iraj Rezaee, Abbas Rasaee, Mohammad Javad Hosseini, Ahmad Zavaran Tabbaraee, Bahman Ghafurian, Sobhan Abdulamir, Ahmed Sahib Abu Bakar, Fatimah Mohammad, Raftari Lipopolysaccharide (LPS) of Brucella abortus is an essential component for developing the subunit vaccine against brucellosis. B. abortus LPS was extracted by n-butanol, purified by ultracentrifugation and detoxified by alkaline treatment. Pyrogenicity and toxicity of B. abortus LPS and detoxified–LPS (D-LPS) were analyzed and compared with LPS of E. coli. Different groups of mice were immunized intraperitoneally with purified B. abortus LPS, D-LPS, a combination of LPS with human serum albumin (LPS-HSA) and B. abortus S19 bacteria; besides, control mice were inoculated with sterile saline. Two doses of vaccine were given 4 weeks apart. Mice were challenged intraperitoneally with virulent B. abortus 544 strain 4 weeks after the second dose of vaccine. Sera and spleens of mice were harvested 4 weeks after challenge. LPS-B. abortus was 10,000-fold less potent in LAL test and 100-fold less potent in eliciting fever in rabbits than in E. coli LPS. And D-LPS was very less potent in LAL test and eliciting fever in rabbits ordinary LPS. The antibody titer of anti-LPS immunoglobulin G (IgG) was higher than D-LPS. However, mice immunized with either LPS, D-LPS or LPS-HSA vaccines showed a significant protection against infection of the spleen (p<0.01). There was no significant difference between mice immunized with LPS and D-LPS in terms of protection (p<0.99). Therefore, it was concluded that D-LPS and LPS-HSA for B. abortus can be used as safer and more potent vaccines than ordinary LPS-B. abortus vaccine. Asian Network for Scientific Information (ANSINET) 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15842/1/Novel%20approach%20of%20vaccination%20against%20Brucella%20abortus%20544%20based%20on%20a%20combination%20of%20fusion%20proteins.pdf Pakzad, Iraj and Rezaee, Abbas and Rasaee, Mohammad Javad and Hosseini, Ahmad Zavaran and Tabbaraee, Bahman and Ghafurian, Sobhan and Abdulamir, Ahmed Sahib and Abu Bakar, Fatimah and Mohammad, Raftari (2010) Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides. Journal of Biological Sciences, 10 (8). pp. 767-772. ISSN 1727-3078 http://scialert.net/abstract/?doi=jbs.2010.767.772 10.3923/jbs.2010.767.772 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Lipopolysaccharide (LPS) of Brucella abortus is an essential component for developing the subunit vaccine against brucellosis. B. abortus LPS was extracted by n-butanol, purified by ultracentrifugation and detoxified by alkaline treatment. Pyrogenicity and toxicity of B. abortus LPS and detoxified–LPS (D-LPS) were analyzed and compared with LPS of E. coli. Different groups of mice were immunized intraperitoneally with purified B. abortus LPS, D-LPS, a combination of LPS with human serum albumin (LPS-HSA) and B. abortus S19 bacteria; besides, control mice were inoculated with sterile saline. Two doses of vaccine were given 4 weeks apart. Mice were challenged intraperitoneally with virulent B. abortus 544 strain 4 weeks after the second dose of vaccine. Sera and spleens of mice were harvested 4 weeks after challenge. LPS-B. abortus was 10,000-fold less potent in LAL test and 100-fold less potent in eliciting fever in rabbits than in E. coli LPS. And D-LPS was very less potent in LAL test and eliciting fever in rabbits ordinary LPS. The antibody titer of anti-LPS immunoglobulin G (IgG) was higher than D-LPS. However, mice immunized with either LPS, D-LPS or LPS-HSA vaccines showed a significant protection against infection of the spleen (p<0.01). There was no significant difference between mice immunized with LPS and D-LPS in terms of protection (p<0.99). Therefore, it was concluded that D-LPS and LPS-HSA for B. abortus can be used as safer and more potent vaccines than ordinary LPS-B. abortus vaccine.
format Article
author Pakzad, Iraj
Rezaee, Abbas
Rasaee, Mohammad Javad
Hosseini, Ahmad Zavaran
Tabbaraee, Bahman
Ghafurian, Sobhan
Abdulamir, Ahmed Sahib
Abu Bakar, Fatimah
Mohammad, Raftari
spellingShingle Pakzad, Iraj
Rezaee, Abbas
Rasaee, Mohammad Javad
Hosseini, Ahmad Zavaran
Tabbaraee, Bahman
Ghafurian, Sobhan
Abdulamir, Ahmed Sahib
Abu Bakar, Fatimah
Mohammad, Raftari
Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.
author_facet Pakzad, Iraj
Rezaee, Abbas
Rasaee, Mohammad Javad
Hosseini, Ahmad Zavaran
Tabbaraee, Bahman
Ghafurian, Sobhan
Abdulamir, Ahmed Sahib
Abu Bakar, Fatimah
Mohammad, Raftari
author_sort Pakzad, Iraj
title Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.
title_short Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.
title_full Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.
title_fullStr Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.
title_full_unstemmed Novel approach of vaccination against Brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus Lipopolysaccharides.
title_sort novel approach of vaccination against brucella abortus 544 based on a combination of fusion proteins, human serum albumin and brucella abortus lipopolysaccharides.
publisher Asian Network for Scientific Information (ANSINET)
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/15842/1/Novel%20approach%20of%20vaccination%20against%20Brucella%20abortus%20544%20based%20on%20a%20combination%20of%20fusion%20proteins.pdf
http://psasir.upm.edu.my/id/eprint/15842/
http://scialert.net/abstract/?doi=jbs.2010.767.772
_version_ 1643826045113073664
score 13.211869

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