Staff View: Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.

Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.

Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph prote...

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Main Authors:	Abdullah Al-Haj, Nagi Ahmed, Shamsudin, Mariana Nor, Abdul Rahim, Raha, Ishak, Zamri
Format:	Article
Language:	English English
Published:	Medwell Publishing 2008
Online Access:	http://psasir.upm.edu.my/id/eprint/15700/1/Molecular%20characterization%20of%20horseshoe%20crab%20anti.pdf http://psasir.upm.edu.my/id/eprint/15700/
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spelling	my.upm.eprints.157002015-12-03T09:04:51Z http://psasir.upm.edu.my/id/eprint/15700/ Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph protein of horseshoe crabs (Factor C anti-LPS) Tachypleus tridentatus that is believed to be involved in the binds to the lipopolysaccharide of Escherichia coli, Salmonella and Vibrio cholerae. The aim of our study the exploit part of cell wall polysaccharide in the development of improved detection method based on molecular approaches. In the gene detection assay, Lipopolysaccharide gene of Salmonella, V. cholera and E. coil were hybridized to anti-LPS factor gene found in the biolysate of the marine animals. The wzm and wzt genes encoding O-polysaccharide genes were amplified in these pathogens and the LPS factor C were amplified from the marine lysate. Development of a PCR-based technique for detection of the food-borne pathogens particularly Sa Salmonella, V. cholera and E. coil were achieved. Thus rapid, sensitive and reliable techniques for the detection of food-borne pathogens developed. Medwell Publishing 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15700/1/Molecular%20characterization%20of%20horseshoe%20crab%20anti.pdf Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. Research Journal of Biological Sciences, 3 (12). pp. 1392-1397. ISSN 1815-8846 rjbsci.2008.1392.1397 English
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English English
description	Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph protein of horseshoe crabs (Factor C anti-LPS) Tachypleus tridentatus that is believed to be involved in the binds to the lipopolysaccharide of Escherichia coli, Salmonella and Vibrio cholerae. The aim of our study the exploit part of cell wall polysaccharide in the development of improved detection method based on molecular approaches. In the gene detection assay, Lipopolysaccharide gene of Salmonella, V. cholera and E. coil were hybridized to anti-LPS factor gene found in the biolysate of the marine animals. The wzm and wzt genes encoding O-polysaccharide genes were amplified in these pathogens and the LPS factor C were amplified from the marine lysate. Development of a PCR-based technique for detection of the food-borne pathogens particularly Sa Salmonella, V. cholera and E. coil were achieved. Thus rapid, sensitive and reliable techniques for the detection of food-borne pathogens developed.
format	Article
author	Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri
spellingShingle	Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
author_facet	Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri
author_sort	Abdullah Al-Haj, Nagi Ahmed
title	Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
title_short	Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
title_full	Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
title_fullStr	Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
title_full_unstemmed	Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
title_sort	molecular characterization of horseshoe crab anti-lipopolysaccharide factor c-peptide for hybridization-based detection method of gram negative bacteria.
publisher	Medwell Publishing
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/15700/1/Molecular%20characterization%20of%20horseshoe%20crab%20anti.pdf http://psasir.upm.edu.my/id/eprint/15700/
_version_	1643826004498579456
score	13.211869

Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.

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