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Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.

A large quantity of ESTs is available from various cDNA libraries of oil palm. The information from oil palm EST databases has been utilized to identify several interesting sequences for molecular characterization. In this study, we report molecular characterization of clone 583 (Acc. No. EU795363)...

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Main Authors: Le, Vinh Thuc, Huynh, Ky, Siew, Eng Ooi, Napis, Suhaimi, Ishak, Zamzuri, Namasivayam, Parameswari
Format: Article
Language:English
English
Published: Universiti Putra Malaysia 2008
Online Access:http://psasir.upm.edu.my/id/eprint/15698/1/Molecular%20characterization%20of%20an%20unknown%20protein.pdf
http://psasir.upm.edu.my/id/eprint/15698/
http://www.pertanika.upm.edu.my/JST.php
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spelling my.upm.eprints.156982015-10-05T07:50:36Z http://psasir.upm.edu.my/id/eprint/15698/ Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. Le, Vinh Thuc Huynh, Ky Siew, Eng Ooi Napis, Suhaimi Ishak, Zamzuri Namasivayam, Parameswari A large quantity of ESTs is available from various cDNA libraries of oil palm. The information from oil palm EST databases has been utilized to identify several interesting sequences for molecular characterization. In this study, we report molecular characterization of clone 583 (Acc. No. EU795363) isolated from cell suspension culture of oil palm. This clone is predicted to encode a single major open reading frame for a polypeptide of 177 amino acids with a predicted molecular mass of 19.6 kDa. The predicted amino acid sequence does not contain any signal peptide and transmembrane region. Based on Kyte-Doolittle hydropathy profile, this protein is predicted to be a soluble protein. The predicted ORF of clone 583 was 59% identical to an amino acid sequence of an unknown protein from Oryza sativa (Acc. BAD25663). Southern analysis showed that this clone might be a member of a multigene family in the oil palm genome. Gene expression study by real time quantitative RT-PCR showed that transcripts of clone 583 might be present in low abundance. Universiti Putra Malaysia 2008-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15698/1/Molecular%20characterization%20of%20an%20unknown%20protein.pdf Le, Vinh Thuc and Huynh, Ky and Siew, Eng Ooi and Napis, Suhaimi and Ishak, Zamzuri and Namasivayam, Parameswari (2008) Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. Pertanika Journal of Science & Technology, 16 (2). pp. 189-199. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/JST.php English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A large quantity of ESTs is available from various cDNA libraries of oil palm. The information from oil palm EST databases has been utilized to identify several interesting sequences for molecular characterization. In this study, we report molecular characterization of clone 583 (Acc. No. EU795363) isolated from cell suspension culture of oil palm. This clone is predicted to encode a single major open reading frame for a polypeptide of 177 amino acids with a predicted molecular mass of 19.6 kDa. The predicted amino acid sequence does not contain any signal peptide and transmembrane region. Based on Kyte-Doolittle hydropathy profile, this protein is predicted to be a soluble protein. The predicted ORF of clone 583 was 59% identical to an amino acid sequence of an unknown protein from Oryza sativa (Acc. BAD25663). Southern analysis showed that this clone might be a member of a multigene family in the oil palm genome. Gene expression study by real time quantitative RT-PCR showed that transcripts of clone 583 might be present in low abundance.
format Article
author Le, Vinh Thuc
Huynh, Ky
Siew, Eng Ooi
Napis, Suhaimi
Ishak, Zamzuri
Namasivayam, Parameswari
spellingShingle Le, Vinh Thuc
Huynh, Ky
Siew, Eng Ooi
Napis, Suhaimi
Ishak, Zamzuri
Namasivayam, Parameswari
Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
author_facet Le, Vinh Thuc
Huynh, Ky
Siew, Eng Ooi
Napis, Suhaimi
Ishak, Zamzuri
Namasivayam, Parameswari
author_sort Le, Vinh Thuc
title Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
title_short Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
title_full Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
title_fullStr Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
title_full_unstemmed Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
title_sort molecular characterization of an unknown protein (acc. no. eu795363) from the ests of oil palm (elaeis guineensis jacq.) cell suspension culture.
publisher Universiti Putra Malaysia
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/15698/1/Molecular%20characterization%20of%20an%20unknown%20protein.pdf
http://psasir.upm.edu.my/id/eprint/15698/
http://www.pertanika.upm.edu.my/JST.php
_version_ 1643826003901939712
score 13.211869

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