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Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia

Madagascar periwinkle is a common decorative, easy growing and spreading perennial herb. Phyllody, virescence, proliferation, little leaf and yellowing symptoms were observed on periwinkle in Serdang and Banting, Selangor, Malaysia. Polymerase chain reaction (PCR) assays using P1/P7 universal phytop...

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Main Authors: Nejat, Naghmeh, Sijam, Kamaruzaman, Abdullah, Siti Nor Akmar, Vadamalai, Ganesan, Dickinson, Matthew
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15697/1/15697.pdf
http://psasir.upm.edu.my/id/eprint/15697/
http://www.academicjournals.org/journal/AJB/article-abstract/BFC128E20545
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spelling my.upm.eprints.156972017-11-14T08:14:02Z http://psasir.upm.edu.my/id/eprint/15697/ Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia Nejat, Naghmeh Sijam, Kamaruzaman Abdullah, Siti Nor Akmar Vadamalai, Ganesan Dickinson, Matthew Madagascar periwinkle is a common decorative, easy growing and spreading perennial herb. Phyllody, virescence, proliferation, little leaf and yellowing symptoms were observed on periwinkle in Serdang and Banting, Selangor, Malaysia. Polymerase chain reaction (PCR) assays using P1/P7 universal phytoplasma primers and secA primers designed for identification and classification of phytoplasma, amplified 1.8 kb fragment that encompasses the entire 16S rRNA gene, the 16S - 23S intergenic spacer region and the beginning of the 23S rRNA gene as well as the 840 bp from part of secA gene, respectively. Sequence analysis of the 16S rDNA, 16S - 23S intergenic region and the beginning of the 23S rRNA and secA gene PCR products determined that the phytoplasma strain associated with periwinkle proliferation in Malaysia belongs to the ‘CandidatusPhytoplasma asteries’ (16Sr I-B) group of phytoplasmas. The virtual restriction fragment length polymorphism (RFLP) analysis with 10 restriction endonulease enzymes revealed identical patterns to phytoplasmas members of Aster yellows phytoplasma subgroup B. A phylogenetic tree based on 16S rDNA sequences,secA gene sequences and virtual RFLP revealed that the periwinkle proliferation phytoplasma is closely related to the subgroup 16SrI-B. Periwinkle proliferation also confirmed which 16SrI-B has wide geographical distribution and host range. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15697/1/15697.pdf Nejat, Naghmeh and Sijam, Kamaruzaman and Abdullah, Siti Nor Akmar and Vadamalai, Ganesan and Dickinson, Matthew (2010) Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia. African Journal of Biotechnology, 9 (15). art. no. BFC128E20545. pp. 2305-2315. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/BFC128E20545
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Madagascar periwinkle is a common decorative, easy growing and spreading perennial herb. Phyllody, virescence, proliferation, little leaf and yellowing symptoms were observed on periwinkle in Serdang and Banting, Selangor, Malaysia. Polymerase chain reaction (PCR) assays using P1/P7 universal phytoplasma primers and secA primers designed for identification and classification of phytoplasma, amplified 1.8 kb fragment that encompasses the entire 16S rRNA gene, the 16S - 23S intergenic spacer region and the beginning of the 23S rRNA gene as well as the 840 bp from part of secA gene, respectively. Sequence analysis of the 16S rDNA, 16S - 23S intergenic region and the beginning of the 23S rRNA and secA gene PCR products determined that the phytoplasma strain associated with periwinkle proliferation in Malaysia belongs to the ‘CandidatusPhytoplasma asteries’ (16Sr I-B) group of phytoplasmas. The virtual restriction fragment length polymorphism (RFLP) analysis with 10 restriction endonulease enzymes revealed identical patterns to phytoplasmas members of Aster yellows phytoplasma subgroup B. A phylogenetic tree based on 16S rDNA sequences,secA gene sequences and virtual RFLP revealed that the periwinkle proliferation phytoplasma is closely related to the subgroup 16SrI-B. Periwinkle proliferation also confirmed which 16SrI-B has wide geographical distribution and host range.
format Article
author Nejat, Naghmeh
Sijam, Kamaruzaman
Abdullah, Siti Nor Akmar
Vadamalai, Ganesan
Dickinson, Matthew
spellingShingle Nejat, Naghmeh
Sijam, Kamaruzaman
Abdullah, Siti Nor Akmar
Vadamalai, Ganesan
Dickinson, Matthew
Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
author_facet Nejat, Naghmeh
Sijam, Kamaruzaman
Abdullah, Siti Nor Akmar
Vadamalai, Ganesan
Dickinson, Matthew
author_sort Nejat, Naghmeh
title Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
title_short Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
title_full Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
title_fullStr Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
title_full_unstemmed Molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in Malaysia
title_sort molecular characterization of an aster yellows phytoplasma associated with proliferation of periwinkle in malaysia
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/15697/1/15697.pdf
http://psasir.upm.edu.my/id/eprint/15697/
http://www.academicjournals.org/journal/AJB/article-abstract/BFC128E20545
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score 13.211869

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