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Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods

The optimization of fermentation medium for the production of chitinase by Bacillus licheniformis TH-1 was carried out using Response Surface Methodology (RSM) based on the two level factorial design. This procedure limited the number of actual experiments performed while allowing for possible inter...

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Main Authors: Mohd Akhir , Sobri, Abd. Aziz , Suraini, Md Salleh, Madihah, Abdul Rahman, Roshanida, Md Illias, Rosli, Hassan, Mohd Ali
Format: Article
Language:English
English
Published: Asian Network for Scientific Information (ANSINET) 2009
Online Access:http://psasir.upm.edu.my/id/eprint/15598/1/Medium%20optimisation%20of%20chitinase%20enzyme%20production%20from%20shrimp%20waste%20using%20Bacillus%20licheniformis%20TH.pdf
http://psasir.upm.edu.my/id/eprint/15598/
http://scialert.net/abstract/?doi=biotech.2009.120.125
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spelling my.upm.eprints.155982015-10-22T23:37:47Z http://psasir.upm.edu.my/id/eprint/15598/ Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods Mohd Akhir , Sobri Abd. Aziz , Suraini Md Salleh, Madihah Abdul Rahman, Roshanida Md Illias, Rosli Hassan, Mohd Ali The optimization of fermentation medium for the production of chitinase by Bacillus licheniformis TH-1 was carried out using Response Surface Methodology (RSM) based on the two level factorial design. This procedure limited the number of actual experiments performed while allowing for possible interactions between 5 components. RSM was adopted to derive a statistical model for the effect of chitin, Yeast Extract (YE), peptone, NaNO3 and K2HPO4 on chitinase production. The p-value of the coefficient for linear effects of chitin, peptone and YE was 0.0001, suggesting that this was the principal experiment variable, having the greatest effect on the production of chitinase. The optimal combinations of media constituent for maximum chitinase production are determined as 10 g L-1 chitin, 0.5 g L-1 YE, 0.5 g L-1 peptone, 2.55 g L-1 NaNO3 and 1.55 g L-1 K2HPO4. The optimization of the fermentation medium resulted not only in a 5.4 fold increase of enzyme activity compared to unoptimized medium but also a reduced amount of the required medium constituents. The response surface analysis provided a useful tool for the optimization of a low cost enzyme producing medium for potential use on an industrial scale. Asian Network for Scientific Information (ANSINET) 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15598/1/Medium%20optimisation%20of%20chitinase%20enzyme%20production%20from%20shrimp%20waste%20using%20Bacillus%20licheniformis%20TH.pdf Mohd Akhir , Sobri and Abd. Aziz , Suraini and Md Salleh, Madihah and Abdul Rahman, Roshanida and Md Illias, Rosli and Hassan, Mohd Ali (2009) Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods. Biotechnology, 8 (1). pp. 120-125. ISSN 1682-296X http://scialert.net/abstract/?doi=biotech.2009.120.125 DOI: 10.3923/biotech.2009.120.125 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The optimization of fermentation medium for the production of chitinase by Bacillus licheniformis TH-1 was carried out using Response Surface Methodology (RSM) based on the two level factorial design. This procedure limited the number of actual experiments performed while allowing for possible interactions between 5 components. RSM was adopted to derive a statistical model for the effect of chitin, Yeast Extract (YE), peptone, NaNO3 and K2HPO4 on chitinase production. The p-value of the coefficient for linear effects of chitin, peptone and YE was 0.0001, suggesting that this was the principal experiment variable, having the greatest effect on the production of chitinase. The optimal combinations of media constituent for maximum chitinase production are determined as 10 g L-1 chitin, 0.5 g L-1 YE, 0.5 g L-1 peptone, 2.55 g L-1 NaNO3 and 1.55 g L-1 K2HPO4. The optimization of the fermentation medium resulted not only in a 5.4 fold increase of enzyme activity compared to unoptimized medium but also a reduced amount of the required medium constituents. The response surface analysis provided a useful tool for the optimization of a low cost enzyme producing medium for potential use on an industrial scale.
format Article
author Mohd Akhir , Sobri
Abd. Aziz , Suraini
Md Salleh, Madihah
Abdul Rahman, Roshanida
Md Illias, Rosli
Hassan, Mohd Ali
spellingShingle Mohd Akhir , Sobri
Abd. Aziz , Suraini
Md Salleh, Madihah
Abdul Rahman, Roshanida
Md Illias, Rosli
Hassan, Mohd Ali
Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods
author_facet Mohd Akhir , Sobri
Abd. Aziz , Suraini
Md Salleh, Madihah
Abdul Rahman, Roshanida
Md Illias, Rosli
Hassan, Mohd Ali
author_sort Mohd Akhir , Sobri
title Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods
title_short Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods
title_full Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods
title_fullStr Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods
title_full_unstemmed Medium optimisation of chitinase enzyme production from shrimp waste using Bacillus licheniformis TH-1 by response surface methods
title_sort medium optimisation of chitinase enzyme production from shrimp waste using bacillus licheniformis th-1 by response surface methods
publisher Asian Network for Scientific Information (ANSINET)
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/15598/1/Medium%20optimisation%20of%20chitinase%20enzyme%20production%20from%20shrimp%20waste%20using%20Bacillus%20licheniformis%20TH.pdf
http://psasir.upm.edu.my/id/eprint/15598/
http://scialert.net/abstract/?doi=biotech.2009.120.125
_version_ 1643825974711681024
score 13.211869

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