Cover Image

Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent

Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique...

Full description

Saved in:
Bibliographic Details
Main Authors: Ling, Tau Chuan, Lyddiatt, Andrew
Format: Article
Language:English
Published: The Korean Society for Biotechnology and Bioengineering 2006
Online Access:http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf
http://psasir.upm.edu.my/id/eprint/15491/
https://link.springer.com/article/10.1007/BF02931874
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.15491
record_format eprints
spelling my.upm.eprints.154912019-10-04T08:25:05Z http://psasir.upm.edu.my/id/eprint/15491/ Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent Ling, Tau Chuan Lyddiatt, Andrew Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg. The Korean Society for Biotechnology and Bioengineering 2006 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf Ling, Tau Chuan and Lyddiatt, Andrew (2006) Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent. Biotechnology and Bioprocess Engineering, 11 (1). pp. 84-87. ISSN 1226-8372; ESSN: 1976-3816 https://link.springer.com/article/10.1007/BF02931874 10.1007/BF02931874
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg.
format Article
author Ling, Tau Chuan
Lyddiatt, Andrew
spellingShingle Ling, Tau Chuan
Lyddiatt, Andrew
Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
author_facet Ling, Tau Chuan
Lyddiatt, Andrew
author_sort Ling, Tau Chuan
title Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
title_short Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
title_full Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
title_fullStr Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
title_full_unstemmed Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
title_sort effect of polymer shielding on elution of g3pdh bound to dye-ligand adsorbent
publisher The Korean Society for Biotechnology and Bioengineering
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf
http://psasir.upm.edu.my/id/eprint/15491/
https://link.springer.com/article/10.1007/BF02931874
_version_ 1648738070379364352
score 13.211869

Similar Items