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Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture

Problem statement: Interest in xylanase enzyme application has markedly increased in pulp and paper processing industries. The switch to xylanase-producing recombinant Escherichia coli DH5α pTP510 is seen here as an economic alternative towards higher productivity and easier downstream purification....

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Main Authors: Mohd Rusli, Farliahati, Mohamed, Mohd Shamzi, Mohamed, Rosfarizan, Puspaningsih, Ni Nyoman Tri, Ariff, Arbakariya
Format: Article
Language:English
Published: Science Publications 2009
Online Access:http://psasir.upm.edu.my/id/eprint/15391/1/ajbbsp.2009.109.117.pdf
http://psasir.upm.edu.my/id/eprint/15391/
http://thescipub.com/abstract/10.3844/ajbbsp.2009.109.117
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spelling my.upm.eprints.153912017-12-04T04:46:12Z http://psasir.upm.edu.my/id/eprint/15391/ Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture Mohd Rusli, Farliahati Mohamed, Mohd Shamzi Mohamed, Rosfarizan Puspaningsih, Ni Nyoman Tri Ariff, Arbakariya Problem statement: Interest in xylanase enzyme application has markedly increased in pulp and paper processing industries. The switch to xylanase-producing recombinant Escherichia coli DH5α pTP510 is seen here as an economic alternative towards higher productivity and easier downstream purification. Modeling of E. coli DH5α growth and enzyme secretion is thus desired for future optimization in fermentation process. Approach: Kinetics of intracellular xylanase fermentation by a recombinant E. coli DH5α was studied in shake flask culture. The effect of different medium formulations (complex, minimal and defined), initial pH (6.5, 7.0, 7.4 and 8.0) and agitation speeds (150, 200 and 250 rpm) on cell growth and xylanase production were evaluated. Mathematical models based on Logistic and Luedeking-Piret equations had been proposed to describe the microbial growth and xylanase production. Results: Highest xylanase production was obtained in defined medium. Based on medium formulation, the highest cell concentration (4.59 g L-1) and xylanase production (2, 122.5 U mL-1) was obtained when (NH4)2HPO4 was used as the main nitrogen source, with an adjustment of the initial pH to 7.4 and agitation speed of 200 rpm. The maximum specific growth rate (µmax), growth associated xylanase production coefficient (α) and non-growth associated xylanase production coefficient (β) was 0.41 h-1, 474.26 U mg cell-1and 0 U mg cell-1 h-1, respectively. Conclusion: Xylanase production was growth associated process and the enzyme secretion was greatly dependent on cell concentration and the specific growth rate of E. coli DH5α. Science Publications 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15391/1/ajbbsp.2009.109.117.pdf Mohd Rusli, Farliahati and Mohamed, Mohd Shamzi and Mohamed, Rosfarizan and Puspaningsih, Ni Nyoman Tri and Ariff, Arbakariya (2009) Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture. American Journal of Biochemistry and Biotechnology, 5 (3). pp. 109-117. ISSN 1553-3468; ESSN: 1558-6332 http://thescipub.com/abstract/10.3844/ajbbsp.2009.109.117 10.3844/ajbbsp.2009.109.117
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Problem statement: Interest in xylanase enzyme application has markedly increased in pulp and paper processing industries. The switch to xylanase-producing recombinant Escherichia coli DH5α pTP510 is seen here as an economic alternative towards higher productivity and easier downstream purification. Modeling of E. coli DH5α growth and enzyme secretion is thus desired for future optimization in fermentation process. Approach: Kinetics of intracellular xylanase fermentation by a recombinant E. coli DH5α was studied in shake flask culture. The effect of different medium formulations (complex, minimal and defined), initial pH (6.5, 7.0, 7.4 and 8.0) and agitation speeds (150, 200 and 250 rpm) on cell growth and xylanase production were evaluated. Mathematical models based on Logistic and Luedeking-Piret equations had been proposed to describe the microbial growth and xylanase production. Results: Highest xylanase production was obtained in defined medium. Based on medium formulation, the highest cell concentration (4.59 g L-1) and xylanase production (2, 122.5 U mL-1) was obtained when (NH4)2HPO4 was used as the main nitrogen source, with an adjustment of the initial pH to 7.4 and agitation speed of 200 rpm. The maximum specific growth rate (µmax), growth associated xylanase production coefficient (α) and non-growth associated xylanase production coefficient (β) was 0.41 h-1, 474.26 U mg cell-1and 0 U mg cell-1 h-1, respectively. Conclusion: Xylanase production was growth associated process and the enzyme secretion was greatly dependent on cell concentration and the specific growth rate of E. coli DH5α.
format Article
author Mohd Rusli, Farliahati
Mohamed, Mohd Shamzi
Mohamed, Rosfarizan
Puspaningsih, Ni Nyoman Tri
Ariff, Arbakariya
spellingShingle Mohd Rusli, Farliahati
Mohamed, Mohd Shamzi
Mohamed, Rosfarizan
Puspaningsih, Ni Nyoman Tri
Ariff, Arbakariya
Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture
author_facet Mohd Rusli, Farliahati
Mohamed, Mohd Shamzi
Mohamed, Rosfarizan
Puspaningsih, Ni Nyoman Tri
Ariff, Arbakariya
author_sort Mohd Rusli, Farliahati
title Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture
title_short Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture
title_full Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture
title_fullStr Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture
title_full_unstemmed Kinetics of xylanase fermentation by recombinant Escherichia coli DH5α in shake flask culture
title_sort kinetics of xylanase fermentation by recombinant escherichia coli dh5α in shake flask culture
publisher Science Publications
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/15391/1/ajbbsp.2009.109.117.pdf
http://psasir.upm.edu.my/id/eprint/15391/
http://thescipub.com/abstract/10.3844/ajbbsp.2009.109.117
_version_ 1643825920657588224
score 13.211869

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