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Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics

Microalgae are promising candidate resources from marine ecology for health-improving effects. Metabolite profiling of the microalgal diatom, Chaetoceros calcitrans was conducted by using robust metabolomics tools, namely 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate dat...

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Main Authors: Azizan, Awanis, Ahamad Bustamam, Muhammad Safwan, Maulidiani, M., Shaari, Khozirah, Ismail, Intan Safinar, Nagao, Norio, Abas, Faridah
Format: Article
Language:English
Published: MDPI 2018
Online Access:http://psasir.upm.edu.my/id/eprint/15351/1/15351.pdf
http://psasir.upm.edu.my/id/eprint/15351/
https://www.mdpi.com/1660-3397/16/5/154
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spelling my.upm.eprints.153512019-07-08T07:30:21Z http://psasir.upm.edu.my/id/eprint/15351/ Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics Azizan, Awanis Ahamad Bustamam, Muhammad Safwan Maulidiani, M. Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah Microalgae are promising candidate resources from marine ecology for health-improving effects. Metabolite profiling of the microalgal diatom, Chaetoceros calcitrans was conducted by using robust metabolomics tools, namely 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate data analysis (MVDA). The unsupervised data analysis, using principal component analysis (PCA), resolved the five types of extracts made by solvents ranging from polar to non-polar into five different clusters. Collectively, with various extraction solvents, 11 amino acids, cholesterol, 6 fatty acids, 2 sugars, 1 osmolyte, 6 carotenoids and 2 chlorophyll pigments were identified. The fatty acids and both carotenoid pigments as well as chlorophyll, were observed in the extracts made from medium polar (acetone, chloroform) and non-polar (hexane) solvents. It is suggested that the compounds were the characteristic markers that influenced the separation between the clusters. Based on partial least square (PLS) analysis, fucoxanthin, astaxanthin, violaxanthin, zeaxanthin, canthaxanthin, and lutein displayed strong correlation to 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and nitric oxide (NO) inhibitory activity. This metabolomics study showed that solvent extractions are one of the main bottlenecks for the maximum recovery of bioactive microalgal compounds and could be a better source of natural antioxidants due to a high value of metabolites. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15351/1/15351.pdf Azizan, Awanis and Ahamad Bustamam, Muhammad Safwan and Maulidiani, M. and Shaari, Khozirah and Ismail, Intan Safinar and Nagao, Norio and Abas, Faridah (2018) Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics. Marine Drugs, 16 (5). art. no. 154. pp. 1-19. ISSN 1660-3397 https://www.mdpi.com/1660-3397/16/5/154 10.3390/md16050154
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Microalgae are promising candidate resources from marine ecology for health-improving effects. Metabolite profiling of the microalgal diatom, Chaetoceros calcitrans was conducted by using robust metabolomics tools, namely 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate data analysis (MVDA). The unsupervised data analysis, using principal component analysis (PCA), resolved the five types of extracts made by solvents ranging from polar to non-polar into five different clusters. Collectively, with various extraction solvents, 11 amino acids, cholesterol, 6 fatty acids, 2 sugars, 1 osmolyte, 6 carotenoids and 2 chlorophyll pigments were identified. The fatty acids and both carotenoid pigments as well as chlorophyll, were observed in the extracts made from medium polar (acetone, chloroform) and non-polar (hexane) solvents. It is suggested that the compounds were the characteristic markers that influenced the separation between the clusters. Based on partial least square (PLS) analysis, fucoxanthin, astaxanthin, violaxanthin, zeaxanthin, canthaxanthin, and lutein displayed strong correlation to 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and nitric oxide (NO) inhibitory activity. This metabolomics study showed that solvent extractions are one of the main bottlenecks for the maximum recovery of bioactive microalgal compounds and could be a better source of natural antioxidants due to a high value of metabolites.
format Article
author Azizan, Awanis
Ahamad Bustamam, Muhammad Safwan
Maulidiani, M.
Shaari, Khozirah
Ismail, Intan Safinar
Nagao, Norio
Abas, Faridah
spellingShingle Azizan, Awanis
Ahamad Bustamam, Muhammad Safwan
Maulidiani, M.
Shaari, Khozirah
Ismail, Intan Safinar
Nagao, Norio
Abas, Faridah
Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
author_facet Azizan, Awanis
Ahamad Bustamam, Muhammad Safwan
Maulidiani, M.
Shaari, Khozirah
Ismail, Intan Safinar
Nagao, Norio
Abas, Faridah
author_sort Azizan, Awanis
title Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
title_short Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
title_full Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
title_fullStr Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
title_full_unstemmed Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
title_sort metabolite profiling of the microalgal diatom chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1h nmr-based metabolomics
publisher MDPI
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/15351/1/15351.pdf
http://psasir.upm.edu.my/id/eprint/15351/
https://www.mdpi.com/1660-3397/16/5/154
_version_ 1643825908293828608
score 13.211869

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