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Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.

Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Thre...

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Main Authors: Shafiquzzaman, Siddiquee, Tan, Soon Guan, Yusuf, Umi Kalsom
Format: Article
Language:English
Published: Korean Society for Microbiology and Biotechnology (KMB) 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15318/
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spelling my.upm.eprints.153182012-08-01T01:31:38Z http://psasir.upm.edu.my/id/eprint/15318/ Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates. Shafiquzzaman, Siddiquee Tan, Soon Guan Yusuf, Umi Kalsom Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderm a species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species. Korean Society for Microbiology and Biotechnology (KMB) 2010 Article PeerReviewed Shafiquzzaman, Siddiquee and Tan, Soon Guan and Yusuf, Umi Kalsom (2010) Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates. Journal of Microbiology and Biotechnology, 20 (9). pp. 1266-1275. ISSN 1017-7825 doi: 10.4014/jmb.1004.04029 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride, and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total protein patterns were shown to be useful for the detection of three Trichoderm a species. The isozyme and protein data were analyzed using the Nei and Li Dice similarity coefficient for pairwise comparison between individual isolates, species isolate group, and for generating a distance matrix. The UPGMA cluster analysis showed a higher degree of relationship between T. harzianum and T. aureoviride than to T. longibrachiatum. These results suggested that the T. harzianum isolates had high levels of genetic variation compared with the other isolates of Trichoderma species.
format Article
author Shafiquzzaman, Siddiquee
Tan, Soon Guan
Yusuf, Umi Kalsom
spellingShingle Shafiquzzaman, Siddiquee
Tan, Soon Guan
Yusuf, Umi Kalsom
Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.
author_facet Shafiquzzaman, Siddiquee
Tan, Soon Guan
Yusuf, Umi Kalsom
author_sort Shafiquzzaman, Siddiquee
title Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.
title_short Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.
title_full Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.
title_fullStr Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.
title_full_unstemmed Isoenzyme analysis and relationships among three species in Malaysian Trichoderma isolates.
title_sort isoenzyme analysis and relationships among three species in malaysian trichoderma isolates.
publisher Korean Society for Microbiology and Biotechnology (KMB)
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/15318/
_version_ 1643825898348085248
score 13.211869

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