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Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells

The current methods for tumor induction in breast cancer research animal models are time-consuming, hazardous, expensive, sometimes irreproducible and inconvenient. We successfully developed a new, simple and cost-effective method in developing solid mammary gland tumor in female Sprague-Dawley rat...

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Main Authors: Abbasalipourkabirreh, Roghayeh, Dehghan, Arash, Salehzadeh, Aref, Shamsabadi, Fatemeh, Abdullah, Rasedee
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/15194/1/15194.pdf
http://psasir.upm.edu.my/id/eprint/15194/
http://www.ajol.info/index.php/ajb/article/view/82711
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spelling my.upm.eprints.151942016-06-30T03:00:35Z http://psasir.upm.edu.my/id/eprint/15194/ Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells Abbasalipourkabirreh, Roghayeh Dehghan, Arash Salehzadeh, Aref Shamsabadi, Fatemeh Abdullah, Rasedee The current methods for tumor induction in breast cancer research animal models are time-consuming, hazardous, expensive, sometimes irreproducible and inconvenient. We successfully developed a new, simple and cost-effective method in developing solid mammary gland tumor in female Sprague-Dawley rat using LA7 rat mammary tumor cells. Tumors developed in 7- 8 weeks old rats within 6 to 8 days of subcutaneous injection of LA7 cells into the mammary gland pad. Tumor size increased exponentially for four weeks. Histopathology examination confirmed that the induced tumors were adenocarcinomas. Evaluation of blood enzymes showed significantly higher (P < 0.005) serum alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in tumor-bearing than in normal rats. This LA7 cell-induced rat mammary gland tumor model may be useful for studies in breast cancer drug or nutraceutical research and development. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15194/1/15194.pdf Abbasalipourkabirreh, Roghayeh and Dehghan, Arash and Salehzadeh, Aref and Shamsabadi, Fatemeh and Abdullah, Rasedee (2010) Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells. African Journal of Biotechnology, 9 (28). art. no. DA61E8D20585. pp. 4491-4498. ISSN 1684–5315 http://www.ajol.info/index.php/ajb/article/view/82711
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The current methods for tumor induction in breast cancer research animal models are time-consuming, hazardous, expensive, sometimes irreproducible and inconvenient. We successfully developed a new, simple and cost-effective method in developing solid mammary gland tumor in female Sprague-Dawley rat using LA7 rat mammary tumor cells. Tumors developed in 7- 8 weeks old rats within 6 to 8 days of subcutaneous injection of LA7 cells into the mammary gland pad. Tumor size increased exponentially for four weeks. Histopathology examination confirmed that the induced tumors were adenocarcinomas. Evaluation of blood enzymes showed significantly higher (P < 0.005) serum alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in tumor-bearing than in normal rats. This LA7 cell-induced rat mammary gland tumor model may be useful for studies in breast cancer drug or nutraceutical research and development.
format Article
author Abbasalipourkabirreh, Roghayeh
Dehghan, Arash
Salehzadeh, Aref
Shamsabadi, Fatemeh
Abdullah, Rasedee
spellingShingle Abbasalipourkabirreh, Roghayeh
Dehghan, Arash
Salehzadeh, Aref
Shamsabadi, Fatemeh
Abdullah, Rasedee
Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells
author_facet Abbasalipourkabirreh, Roghayeh
Dehghan, Arash
Salehzadeh, Aref
Shamsabadi, Fatemeh
Abdullah, Rasedee
author_sort Abbasalipourkabirreh, Roghayeh
title Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells
title_short Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells
title_full Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells
title_fullStr Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells
title_full_unstemmed Induction of mammary gland tumor in female Sprague-Dawley rats with LA7 cells
title_sort induction of mammary gland tumor in female sprague-dawley rats with la7 cells
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/15194/1/15194.pdf
http://psasir.upm.edu.my/id/eprint/15194/
http://www.ajol.info/index.php/ajb/article/view/82711
_version_ 1643825861725519872
score 13.211869

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