Cover Image

Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.

BACKGROUND: Outbreaks of the casuarina moth, Lymantria xylina Swinehoe (Lepidoptera: Lymantriidae), which is a very important forest pest in Taiwan, have occurred every five to 10 years. This moth has expanded its range of host plants to include more than 65 species of broadleaf trees. LyxyMNPV (L....

Full description

Saved in:
Bibliographic Details
Main Authors: Nai, Yu Shin, Wu, Chih Yu, Wang, Tai Chuan, Chen, Yun Ru, Lau, Wei Hong, Lo, Chu Fang, Tsai, Meng Feng, Wang, Chung Hsiung
Format: Article
Language:English
English
Published: BioMed Central 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14884/1/Genomic%20sequencing%20and%20analyses%20of%20Lymantria%20xylina%20multiple%20nucleopolyhedrovirus.pdf
http://psasir.upm.edu.my/id/eprint/14884/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.14884
record_format eprints
spelling my.upm.eprints.148842015-12-16T08:00:26Z http://psasir.upm.edu.my/id/eprint/14884/ Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus. Nai, Yu Shin Wu, Chih Yu Wang, Tai Chuan Chen, Yun Ru Lau, Wei Hong Lo, Chu Fang Tsai, Meng Feng Wang, Chung Hsiung BACKGROUND: Outbreaks of the casuarina moth, Lymantria xylina Swinehoe (Lepidoptera: Lymantriidae), which is a very important forest pest in Taiwan, have occurred every five to 10 years. This moth has expanded its range of host plants to include more than 65 species of broadleaf trees. LyxyMNPV (L. xylina multiple nucleopolyhedrovirus) is highly virulent to the casuarina moth and has been investigated as a possible biopesticide for controlling this moth. LdMNPV-like virus has also been isolated from Lymantria xylina larvae but LyxyMNPV was more virulent than LdMNPV-like virus both in NTU-LY and IPLB-LD-652Y cell lines. To better understand LyxyMNPV, the nucleotide sequence of the LyxyMNPV DNA genome was determined and analysed. RESULTS: The genome of LyxyMNPV consists of 156,344 bases, has a G+C content of 53.4% and contains 157 putative open reading frames (ORFs). The gene content and gene order of LyxyMNPV were similar to those of LdMNPV, with 151 ORFs identified as homologous to those reported in the LdMNPV genome. Two genes (Lyxy49 and Lyxy123) were homologous to other baculoviruses, and four unique LyxyMNPV ORFs (Lyxy11, Lyxy19, Lyxy130 and Lyxy131) were identified in the LyxyMNPV genome, including a gag-like gene that was not reported in baculoviruses. LdMNPV contains 23 ORFs that are absent in LyxyMNPV. Readily identifiable homologues of the gene host range factor-1 (hrf-1), which appears to be involved in the susceptibility of L. dispar to NPV infection, were not present in LyxyMNPV. Additionally, two putative odv-e27 homologues were identified in LyxyMNPV. The LyxyMNPV genome encoded 14 bro genes compared with 16 in LdMNPV, which occupied more than 8% of the LyxyMNPV genome. Thirteen homologous regions (hrs) were identified containing 48 repeated sequences composed of 30-bp imperfect palindromes. However, they differed in the relative positions, number of repeats and orientation in the genome compared to LdMNPV. CONCLUSION: The gene parity plot analysis, percent identity of the gene homologues and a phylogenetic analysis suggested that LyxyMNPV is a Group II NPV that is most closely related to LdMNPV but with a highly distinct genomic organisation. BioMed Central 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14884/1/Genomic%20sequencing%20and%20analyses%20of%20Lymantria%20xylina%20multiple%20nucleopolyhedrovirus.pdf Nai, Yu Shin and Wu, Chih Yu and Wang, Tai Chuan and Chen, Yun Ru and Lau, Wei Hong and Lo, Chu Fang and Tsai, Meng Feng and Wang, Chung Hsiung (2010) Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus. BMC Genomics , 11. 1- 14. ISSN 1471-2164 10.1186/1471-2164-11-116 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description BACKGROUND: Outbreaks of the casuarina moth, Lymantria xylina Swinehoe (Lepidoptera: Lymantriidae), which is a very important forest pest in Taiwan, have occurred every five to 10 years. This moth has expanded its range of host plants to include more than 65 species of broadleaf trees. LyxyMNPV (L. xylina multiple nucleopolyhedrovirus) is highly virulent to the casuarina moth and has been investigated as a possible biopesticide for controlling this moth. LdMNPV-like virus has also been isolated from Lymantria xylina larvae but LyxyMNPV was more virulent than LdMNPV-like virus both in NTU-LY and IPLB-LD-652Y cell lines. To better understand LyxyMNPV, the nucleotide sequence of the LyxyMNPV DNA genome was determined and analysed. RESULTS: The genome of LyxyMNPV consists of 156,344 bases, has a G+C content of 53.4% and contains 157 putative open reading frames (ORFs). The gene content and gene order of LyxyMNPV were similar to those of LdMNPV, with 151 ORFs identified as homologous to those reported in the LdMNPV genome. Two genes (Lyxy49 and Lyxy123) were homologous to other baculoviruses, and four unique LyxyMNPV ORFs (Lyxy11, Lyxy19, Lyxy130 and Lyxy131) were identified in the LyxyMNPV genome, including a gag-like gene that was not reported in baculoviruses. LdMNPV contains 23 ORFs that are absent in LyxyMNPV. Readily identifiable homologues of the gene host range factor-1 (hrf-1), which appears to be involved in the susceptibility of L. dispar to NPV infection, were not present in LyxyMNPV. Additionally, two putative odv-e27 homologues were identified in LyxyMNPV. The LyxyMNPV genome encoded 14 bro genes compared with 16 in LdMNPV, which occupied more than 8% of the LyxyMNPV genome. Thirteen homologous regions (hrs) were identified containing 48 repeated sequences composed of 30-bp imperfect palindromes. However, they differed in the relative positions, number of repeats and orientation in the genome compared to LdMNPV. CONCLUSION: The gene parity plot analysis, percent identity of the gene homologues and a phylogenetic analysis suggested that LyxyMNPV is a Group II NPV that is most closely related to LdMNPV but with a highly distinct genomic organisation.
format Article
author Nai, Yu Shin
Wu, Chih Yu
Wang, Tai Chuan
Chen, Yun Ru
Lau, Wei Hong
Lo, Chu Fang
Tsai, Meng Feng
Wang, Chung Hsiung
spellingShingle Nai, Yu Shin
Wu, Chih Yu
Wang, Tai Chuan
Chen, Yun Ru
Lau, Wei Hong
Lo, Chu Fang
Tsai, Meng Feng
Wang, Chung Hsiung
Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.
author_facet Nai, Yu Shin
Wu, Chih Yu
Wang, Tai Chuan
Chen, Yun Ru
Lau, Wei Hong
Lo, Chu Fang
Tsai, Meng Feng
Wang, Chung Hsiung
author_sort Nai, Yu Shin
title Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.
title_short Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.
title_full Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.
title_fullStr Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.
title_full_unstemmed Genomic sequencing and analyses of Lymantria xylina multiple nucleopolyhedrovirus.
title_sort genomic sequencing and analyses of lymantria xylina multiple nucleopolyhedrovirus.
publisher BioMed Central
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/14884/1/Genomic%20sequencing%20and%20analyses%20of%20Lymantria%20xylina%20multiple%20nucleopolyhedrovirus.pdf
http://psasir.upm.edu.my/id/eprint/14884/
_version_ 1643825768351924224
score 13.211869

Similar Items