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Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.

A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cel...

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Main Authors: Abdullah, Syahrilnizam, Wai Yeng, Wendy-Yeo, Hosseinkhani, Hossein, Hosseinkhani, Mohsen, Masrawa, Ehab, Ramasamy, Rajesh, Rosli, Rozita, Abdul Rahman, Sabariah, Domb, Abraham J.
Format: Article
Language:English
English
Published: Hindawi Publishing Corporation 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14867/1/Gene%20transfer%20into%20the%20lung%20by%20nanoparticle%20dextran.pdf
http://psasir.upm.edu.my/id/eprint/14867/
http://www.hindawi.com/
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spelling my.upm.eprints.148672015-10-29T07:38:28Z http://psasir.upm.edu.my/id/eprint/14867/ Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes. Abdullah, Syahrilnizam Wai Yeng, Wendy-Yeo Hosseinkhani, Hossein Hosseinkhani, Mohsen Masrawa, Ehab Ramasamy, Rajesh Rosli, Rozita Abdul Rahman, Sabariah Domb, Abraham J. A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery. In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range. Hindawi Publishing Corporation 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14867/1/Gene%20transfer%20into%20the%20lung%20by%20nanoparticle%20dextran.pdf Abdullah, Syahrilnizam and Wai Yeng, Wendy-Yeo and Hosseinkhani, Hossein and Hosseinkhani, Mohsen and Masrawa, Ehab and Ramasamy, Rajesh and Rosli, Rozita and Abdul Rahman, Sabariah and Domb, Abraham J. (2010) Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes. Journal of Biomedicine and Biotechnology, 2010 (284840). pp. 1-10. ISSN 1110-7243, ESSN: 1110-7251 http://www.hindawi.com/ 10.1155/2010/284840 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A novel cationic polymer, dextran-spermine (D-SPM), has been found to mediate gene expression in a wide variety of cell lines and in vivo through systemic delivery. Here, we extended the observations by determining the optimal conditions for gene expression of D-SPM/plasmid DNA (D-SPM/pDNA) in cell lines and in the lungs of BALB/c mice via instillation delivery. In vitro studies showed that D-SPM could partially protect pDNA from degradation by nuclease and exhibited optimal gene transfer efficiency at D-SPM to pDNA weight-mixing ratio of 12. In the lungs of mice, the levels of gene expression generated by D-SPM/pDNA are highly dependent on the weight-mixing ratio of D-SPM to pDNA, amount of pDNA in the complex, and the assay time postdelivery. Readministration of the complex at day 1 following the first dosing showed no significant effect on the retention and duration of gene expression. The study also showed that there was a clear trend of increasing size of the complexes as the amount of pDNA was increased, where the sizes of the D-SPM/pDNA complexes were within the nanometer range.
format Article
author Abdullah, Syahrilnizam
Wai Yeng, Wendy-Yeo
Hosseinkhani, Hossein
Hosseinkhani, Mohsen
Masrawa, Ehab
Ramasamy, Rajesh
Rosli, Rozita
Abdul Rahman, Sabariah
Domb, Abraham J.
spellingShingle Abdullah, Syahrilnizam
Wai Yeng, Wendy-Yeo
Hosseinkhani, Hossein
Hosseinkhani, Mohsen
Masrawa, Ehab
Ramasamy, Rajesh
Rosli, Rozita
Abdul Rahman, Sabariah
Domb, Abraham J.
Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
author_facet Abdullah, Syahrilnizam
Wai Yeng, Wendy-Yeo
Hosseinkhani, Hossein
Hosseinkhani, Mohsen
Masrawa, Ehab
Ramasamy, Rajesh
Rosli, Rozita
Abdul Rahman, Sabariah
Domb, Abraham J.
author_sort Abdullah, Syahrilnizam
title Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
title_short Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
title_full Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
title_fullStr Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
title_full_unstemmed Gene transfer into the lung by nanoparticle dextran-spermine/plasmid DNA complexes.
title_sort gene transfer into the lung by nanoparticle dextran-spermine/plasmid dna complexes.
publisher Hindawi Publishing Corporation
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/14867/1/Gene%20transfer%20into%20the%20lung%20by%20nanoparticle%20dextran.pdf
http://psasir.upm.edu.my/id/eprint/14867/
http://www.hindawi.com/
_version_ 1643825763281010688
score 13.211869

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