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Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.

An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide com...

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Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Ahmad Kamarudin, Nor Hafizah, Yunus, Jalimah, Salleh, Abu Bakar, Basri, Mahiran
Format: Article
Language:English
English
Published: MDPI Publishing 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14671/1/Expression%20of%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf
http://psasir.upm.edu.my/id/eprint/14671/
http://www.mdpi.com/journal/ijms
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spelling my.upm.eprints.146712015-11-02T02:24:28Z http://psasir.upm.edu.my/id/eprint/14671/ Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. Raja Abdul Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Yunus, Jalimah Salleh, Abu Bakar Basri, Mahiran An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids. Expression of AT2 lipase resulted in an 18-fold increase in activity, upon the induction of 0.6 mM IPTG after a 10 h incubation period. Interestingly, this lipase was stable in various organic solvents (25% (v/v), mainly toluene, octanol, p-xylene and n-hexane). Literature shows that most of the organic solvent stable bacterial lipases were produced by Pseudomonas sp. and Bacillus sp., but very few from Staphylococcus sp. This lipase demonstrates great potential to be employed in various industrial applications. MDPI Publishing 2010-09-13 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14671/1/Expression%20of%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf Raja Abdul Rahman, Raja Noor Zaliha and Ahmad Kamarudin, Nor Hafizah and Yunus, Jalimah and Salleh, Abu Bakar and Basri, Mahiran (2010) Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2. International Journal of Molecular Sciences, 11. pp. 3195-3208. ISSN 1422-0067 http://www.mdpi.com/journal/ijms 10.3390/ijms11093195 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids. Expression of AT2 lipase resulted in an 18-fold increase in activity, upon the induction of 0.6 mM IPTG after a 10 h incubation period. Interestingly, this lipase was stable in various organic solvents (25% (v/v), mainly toluene, octanol, p-xylene and n-hexane). Literature shows that most of the organic solvent stable bacterial lipases were produced by Pseudomonas sp. and Bacillus sp., but very few from Staphylococcus sp. This lipase demonstrates great potential to be employed in various industrial applications.
format Article
author Raja Abdul Rahman, Raja Noor Zaliha
Ahmad Kamarudin, Nor Hafizah
Yunus, Jalimah
Salleh, Abu Bakar
Basri, Mahiran
spellingShingle Raja Abdul Rahman, Raja Noor Zaliha
Ahmad Kamarudin, Nor Hafizah
Yunus, Jalimah
Salleh, Abu Bakar
Basri, Mahiran
Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
author_facet Raja Abdul Rahman, Raja Noor Zaliha
Ahmad Kamarudin, Nor Hafizah
Yunus, Jalimah
Salleh, Abu Bakar
Basri, Mahiran
author_sort Raja Abdul Rahman, Raja Noor Zaliha
title Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
title_short Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
title_full Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
title_fullStr Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
title_full_unstemmed Expression of organic solvent stable lipase from Staphylococcus epidermidis AT2.
title_sort expression of organic solvent stable lipase from staphylococcus epidermidis at2.
publisher MDPI Publishing
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/14671/1/Expression%20of%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf
http://psasir.upm.edu.my/id/eprint/14671/
http://www.mdpi.com/journal/ijms
_version_ 1643825703388446720
score 13.211869

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