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Evaluation of DNA and RNA extraction methods.

This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex...

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Main Authors: Chin Han, Edwin Shiaw, Mohd Sidik, Shiran, Cheah, Yoke Kqueen, Tan, Geok Chin, Abdul Rahman, Sabariah
Format: Article
Language:English
Published: Malaysian Medical Association. 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14600/
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spelling my.upm.eprints.146002013-11-11T03:34:20Z http://psasir.upm.edu.my/id/eprint/14600/ Evaluation of DNA and RNA extraction methods. Chin Han, Edwin Shiaw Mohd Sidik, Shiran Cheah, Yoke Kqueen Tan, Geok Chin Abdul Rahman, Sabariah This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols. Malaysian Medical Association. 2010-06 Article PeerReviewed Chin Han, Edwin Shiaw and Mohd Sidik, Shiran and Cheah, Yoke Kqueen and Tan, Geok Chin and Abdul Rahman, Sabariah (2010) Evaluation of DNA and RNA extraction methods. Medical Journal of Malaysia, 65 (2). pp. 133-137. ISSN 0300-5283 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method (Methods C and D) and one commercial FFPE DNA Extraction kit (Qiagen, Crawley, UK). For RNA extraction, 2 extraction protocols were evaluated including the enzymatic extraction method (Method 1), and Chelex-100 RNA extraction method (Method 2). Results show that the modified enzymatic extraction method (Method A) is an efficient DNA extraction protocol, while for RNA extraction, the enzymatic method (Method 1) and the Chelex-100 RNA extraction method (Method 2) are equally efficient RNA extraction protocols.
format Article
author Chin Han, Edwin Shiaw
Mohd Sidik, Shiran
Cheah, Yoke Kqueen
Tan, Geok Chin
Abdul Rahman, Sabariah
spellingShingle Chin Han, Edwin Shiaw
Mohd Sidik, Shiran
Cheah, Yoke Kqueen
Tan, Geok Chin
Abdul Rahman, Sabariah
Evaluation of DNA and RNA extraction methods.
author_facet Chin Han, Edwin Shiaw
Mohd Sidik, Shiran
Cheah, Yoke Kqueen
Tan, Geok Chin
Abdul Rahman, Sabariah
author_sort Chin Han, Edwin Shiaw
title Evaluation of DNA and RNA extraction methods.
title_short Evaluation of DNA and RNA extraction methods.
title_full Evaluation of DNA and RNA extraction methods.
title_fullStr Evaluation of DNA and RNA extraction methods.
title_full_unstemmed Evaluation of DNA and RNA extraction methods.
title_sort evaluation of dna and rna extraction methods.
publisher Malaysian Medical Association.
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/14600/
_version_ 1643825681654611968
score 13.211869

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