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Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method

The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components t...

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Main Authors: Tan, Joo Shun, Ramanan, Ramakrishnan Nagasundara, Azaman, Siti Nor Ani, Ling, Tau Chuan, Mustafa, Shuhaimi, Ariff, Arbakariya
Format: Article
Language:English
Published: Bentham Open 2009
Online Access:http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf
http://psasir.upm.edu.my/id/eprint/14509/
https://www.benthamopen.com/ABSTRACT/TOBIOTJ-3-117
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spelling my.upm.eprints.145092019-04-08T08:51:01Z http://psasir.upm.edu.my/id/eprint/14509/ Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method Tan, Joo Shun Ramanan, Ramakrishnan Nagasundara Azaman, Siti Nor Ani Ling, Tau Chuan Mustafa, Shuhaimi Ariff, Arbakariya The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively. Bentham Open 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf Tan, Joo Shun and Ramanan, Ramakrishnan Nagasundara and Azaman, Siti Nor Ani and Ling, Tau Chuan and Mustafa, Shuhaimi and Ariff, Arbakariya (2009) Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method. The Open Biotechnology Journal, 3. pp. 117-124. ISSN 1874-0707 https://www.benthamopen.com/ABSTRACT/TOBIOTJ-3-117 10.2174/1874070700903010117
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively.
format Article
author Tan, Joo Shun
Ramanan, Ramakrishnan Nagasundara
Azaman, Siti Nor Ani
Ling, Tau Chuan
Mustafa, Shuhaimi
Ariff, Arbakariya
spellingShingle Tan, Joo Shun
Ramanan, Ramakrishnan Nagasundara
Azaman, Siti Nor Ani
Ling, Tau Chuan
Mustafa, Shuhaimi
Ariff, Arbakariya
Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
author_facet Tan, Joo Shun
Ramanan, Ramakrishnan Nagasundara
Azaman, Siti Nor Ani
Ling, Tau Chuan
Mustafa, Shuhaimi
Ariff, Arbakariya
author_sort Tan, Joo Shun
title Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
title_short Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
title_full Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
title_fullStr Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
title_full_unstemmed Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method
title_sort enhanced interferon-α2b production in periplasmic space of escherichia coli through medium optimization using response surface method
publisher Bentham Open
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf
http://psasir.upm.edu.my/id/eprint/14509/
https://www.benthamopen.com/ABSTRACT/TOBIOTJ-3-117
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score 13.211869

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