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Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.

Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4), among and within Toxocara canis, T. cati, T. malaysiensis, T. vitulorum and Toxascaris leonina from different geographical origins was e...

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Main Authors: Li, Ming Wei, Lin, Rui Qing, Song, Hui Qun, Abdullah Sani, Rehana, Wu, Xiang Yun, Zhu, Xing Quan
Format: Article
Language:English
English
Published: Wiley-VCH Verlag 2008
Online Access:http://psasir.upm.edu.my/id/eprint/14472/1/Electrophoretic%20analysis%20of%20sequence%20variability%20in%20three%20mitochondrial%20DNA%20regions%20for%20ascaridoid%20parasites%20of%20human%20and%20animal%20health%20significance.pdf
http://psasir.upm.edu.my/id/eprint/14472/
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spelling my.upm.eprints.144722015-10-05T07:49:20Z http://psasir.upm.edu.my/id/eprint/14472/ Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance. Li, Ming Wei Lin, Rui Qing Song, Hui Qun Abdullah Sani, Rehana Wu, Xiang Yun Zhu, Xing Quan Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4), among and within Toxocara canis, T. cati, T. malaysiensis, T. vitulorum and Toxascaris leonina from different geographical origins was examined by a mutation-scanning approach. A portion of the cox1 gene (pcox1), a portion of the nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual ascaridoid nematodes by polymerase chain reaction and the amplicons analyzed by single-strand conformation polymorphism (SSCP). Representative samples displaying sequence variation in SSCP profiles were subjected to sequencing in order to define genetic markers for their specific identification and differentiation. While the intra-specific sequence variations within each of the five ascaridoid species were 0.2-3.7% for pcox1, 0-2.8% for pnad1 and 0-2.3% for pnad4, the inter-specific sequence differences were significantly higher, being 7.9-12.9% for pcox1, 10.7-21.1% for pnad1 and 12.9-21.7% for pnad4, respectively. Phylogenetic analyses based on the combined sequences of pcox1, pnad1 and pnad4 revealed that the recently described species T. malaysiensis was more closely related to T. cati than to T. canis. These findings provided mtDNA evidence for the validity of T. malaysiensis and also demonstrated clearly the usefulness and attributes of the mutation-scanning sequencing approach for studying the population genetic structures of these and other nematodes of socio-economic importance. Wiley-VCH Verlag 2008-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14472/1/Electrophoretic%20analysis%20of%20sequence%20variability%20in%20three%20mitochondrial%20DNA%20regions%20for%20ascaridoid%20parasites%20of%20human%20and%20animal%20health%20significance.pdf Li, Ming Wei and Lin, Rui Qing and Song, Hui Qun and Abdullah Sani, Rehana and Wu, Xiang Yun and Zhu, Xing Quan (2008) Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance. Electrophoresis, 29 (13). pp. 2912-2917. ISSN 0173-0835; ESSN: 1522-2683 10.1002/elps.200700752 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4), among and within Toxocara canis, T. cati, T. malaysiensis, T. vitulorum and Toxascaris leonina from different geographical origins was examined by a mutation-scanning approach. A portion of the cox1 gene (pcox1), a portion of the nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual ascaridoid nematodes by polymerase chain reaction and the amplicons analyzed by single-strand conformation polymorphism (SSCP). Representative samples displaying sequence variation in SSCP profiles were subjected to sequencing in order to define genetic markers for their specific identification and differentiation. While the intra-specific sequence variations within each of the five ascaridoid species were 0.2-3.7% for pcox1, 0-2.8% for pnad1 and 0-2.3% for pnad4, the inter-specific sequence differences were significantly higher, being 7.9-12.9% for pcox1, 10.7-21.1% for pnad1 and 12.9-21.7% for pnad4, respectively. Phylogenetic analyses based on the combined sequences of pcox1, pnad1 and pnad4 revealed that the recently described species T. malaysiensis was more closely related to T. cati than to T. canis. These findings provided mtDNA evidence for the validity of T. malaysiensis and also demonstrated clearly the usefulness and attributes of the mutation-scanning sequencing approach for studying the population genetic structures of these and other nematodes of socio-economic importance.
format Article
author Li, Ming Wei
Lin, Rui Qing
Song, Hui Qun
Abdullah Sani, Rehana
Wu, Xiang Yun
Zhu, Xing Quan
spellingShingle Li, Ming Wei
Lin, Rui Qing
Song, Hui Qun
Abdullah Sani, Rehana
Wu, Xiang Yun
Zhu, Xing Quan
Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.
author_facet Li, Ming Wei
Lin, Rui Qing
Song, Hui Qun
Abdullah Sani, Rehana
Wu, Xiang Yun
Zhu, Xing Quan
author_sort Li, Ming Wei
title Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.
title_short Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.
title_full Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.
title_fullStr Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.
title_full_unstemmed Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance.
title_sort electrophoretic analysis of sequence variability in three mitochondrial dna regions for ascaridoid parasites of human and animal health significance.
publisher Wiley-VCH Verlag
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/14472/1/Electrophoretic%20analysis%20of%20sequence%20variability%20in%20three%20mitochondrial%20DNA%20regions%20for%20ascaridoid%20parasites%20of%20human%20and%20animal%20health%20significance.pdf
http://psasir.upm.edu.my/id/eprint/14472/
_version_ 1643825642942234624
score 13.211869

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