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Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.

Palm-pressed fiber (PPF) is a by-product of palm oil milling and it has been found to contain a high percentage of phospholipids (PL). The aim of this work was to evaluate the best solid-phase extraction (SPE) method to purify PL from PPF. The purified PL were analyzed using high-performance liquid...

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Main Authors: Chua, Seong Chea, Tan, Chin Ping, Lai, Oi Ming, Long, Kamariah, Mirhosseini, Hamed, Baharin, Badlishah Sham
Format: Article
Language:English
English
Published: Wiley 2008
Online Access:http://psasir.upm.edu.my/id/eprint/14197/1/Effect%20of%20absorbent%20in%20solid-phase%20extraction%20.pdf
http://psasir.upm.edu.my/id/eprint/14197/
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spelling my.upm.eprints.141972015-11-23T07:15:01Z http://psasir.upm.edu.my/id/eprint/14197/ Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber. Chua, Seong Chea Tan, Chin Ping Lai, Oi Ming Long, Kamariah Mirhosseini, Hamed Baharin, Badlishah Sham Palm-pressed fiber (PPF) is a by-product of palm oil milling and it has been found to contain a high percentage of phospholipids (PL). The aim of this work was to evaluate the best solid-phase extraction (SPE) method to purify PL from PPF. The purified PL were analyzed using high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector (ELSD). The oil was extracted from PPF using the Folch method and classes of PL were purified using SPE. Different solvent phases and normal-phase SPE cartridges [silica (Si), aminopropyl-bonded silica (NH2) and diol-bonded silica (2OH)] at the same ratio of oil to sorbent mass were used to study the separation of PL from the extracted oil. The recovery of each standard PL was performed in a model oil system with the same amount of standard PL being added, and the repeatability of the SPE method was investigated. The isolation of PL by SPE diol cartridge and subsequent analysis by HPLC/ELSD have shown to be an accurate and reproducible analytical method for the determination of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and lysophosphatidylcholine in PPF. This method provided a high yield and rapid separation of PL in PPF with less contamination from other lipid groups. Wiley 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14197/1/Effect%20of%20absorbent%20in%20solid-phase%20extraction%20.pdf Chua, Seong Chea and Tan, Chin Ping and Lai, Oi Ming and Long, Kamariah and Mirhosseini, Hamed and Baharin, Badlishah Sham (2008) Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber. European Journal of Lipid Science and Technology, 110 (4). pp. 334-340. ISSN 1438 - 7697 10.1002/ejlt.200700179 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Palm-pressed fiber (PPF) is a by-product of palm oil milling and it has been found to contain a high percentage of phospholipids (PL). The aim of this work was to evaluate the best solid-phase extraction (SPE) method to purify PL from PPF. The purified PL were analyzed using high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector (ELSD). The oil was extracted from PPF using the Folch method and classes of PL were purified using SPE. Different solvent phases and normal-phase SPE cartridges [silica (Si), aminopropyl-bonded silica (NH2) and diol-bonded silica (2OH)] at the same ratio of oil to sorbent mass were used to study the separation of PL from the extracted oil. The recovery of each standard PL was performed in a model oil system with the same amount of standard PL being added, and the repeatability of the SPE method was investigated. The isolation of PL by SPE diol cartridge and subsequent analysis by HPLC/ELSD have shown to be an accurate and reproducible analytical method for the determination of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and lysophosphatidylcholine in PPF. This method provided a high yield and rapid separation of PL in PPF with less contamination from other lipid groups.
format Article
author Chua, Seong Chea
Tan, Chin Ping
Lai, Oi Ming
Long, Kamariah
Mirhosseini, Hamed
Baharin, Badlishah Sham
spellingShingle Chua, Seong Chea
Tan, Chin Ping
Lai, Oi Ming
Long, Kamariah
Mirhosseini, Hamed
Baharin, Badlishah Sham
Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
author_facet Chua, Seong Chea
Tan, Chin Ping
Lai, Oi Ming
Long, Kamariah
Mirhosseini, Hamed
Baharin, Badlishah Sham
author_sort Chua, Seong Chea
title Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
title_short Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
title_full Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
title_fullStr Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
title_full_unstemmed Effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
title_sort effect of absorbent in solid-phase extraction on quantification of phospholipids in palm-pressed fiber.
publisher Wiley
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/14197/1/Effect%20of%20absorbent%20in%20solid-phase%20extraction%20.pdf
http://psasir.upm.edu.my/id/eprint/14197/
_version_ 1643825561663963136
score 13.211869

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