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DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.

Electrochemical DNA sensors based on the recognition of a base single- or double-stranded DNA (ss/ds-DNA) sequence specific to a Trichoderma harzianum gene and immobilized onto a gold disk electrode are described here. The ssDNA probe was immobilized on the modified gold electrode to measure the ele...

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Main Authors: Siddiquee, Shafiquzzaman, Yusof, Nor Azah, Salleh, Abu Bakar, Tan, Soon Guan, Abu Bakar, Fatimah, Lee, Yook Heng
Format: Article
Language:English
Published: Elsevier 2010
Online Access:http://psasir.upm.edu.my/id/eprint/14121/
http://www.elsevier.com
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spelling my.upm.eprints.141212013-07-24T02:23:32Z http://psasir.upm.edu.my/id/eprint/14121/ DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode. Siddiquee, Shafiquzzaman Yusof, Nor Azah Salleh, Abu Bakar Tan, Soon Guan Abu Bakar, Fatimah Lee, Yook Heng Electrochemical DNA sensors based on the recognition of a base single- or double-stranded DNA (ss/ds-DNA) sequence specific to a Trichoderma harzianum gene and immobilized onto a gold disk electrode are described here. The ssDNA probe was immobilized on the modified gold electrode to measure the electrochemical response. The hybridization of the probe ssDNA with the target DNA was explored by differential pulse voltammetry (DPV) using methylene blue (MB) as an electroactive indicator. MB on a modified gold electrode was found to be more largely localized than that on a bare gold electrode. Peak currents were found to increase in the order of hybrid-modified-AuE > probe-modified-AuE > non-harzianum > non-complementary DNA. Control experiments with the non-complementary oligonucleotides were performed to assess whether the DNA biosensor responded selectively, via hybridization, to the target. The variation in redox current with the different concentrations of target DNA was linear in the range of 1–20 ppm. Various properties and characteristics of this sensor were also described in this report. A novel approaches to construct an electrochemical biosensor consisting of the probe ssDNA to hybridize with crude DNA fragments from real samples was successfully applied in the study of biological microorganisms. Elsevier 2010 Article PeerReviewed Siddiquee, Shafiquzzaman and Yusof, Nor Azah and Salleh, Abu Bakar and Tan, Soon Guan and Abu Bakar, Fatimah and Lee, Yook Heng (2010) DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode. Sensors and Actuators B: Chemical, 147 (1). pp. 198-205. ISSN 0925-4005 http://www.elsevier.com 10.1016/j.snb.2010.02.014 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Electrochemical DNA sensors based on the recognition of a base single- or double-stranded DNA (ss/ds-DNA) sequence specific to a Trichoderma harzianum gene and immobilized onto a gold disk electrode are described here. The ssDNA probe was immobilized on the modified gold electrode to measure the electrochemical response. The hybridization of the probe ssDNA with the target DNA was explored by differential pulse voltammetry (DPV) using methylene blue (MB) as an electroactive indicator. MB on a modified gold electrode was found to be more largely localized than that on a bare gold electrode. Peak currents were found to increase in the order of hybrid-modified-AuE > probe-modified-AuE > non-harzianum > non-complementary DNA. Control experiments with the non-complementary oligonucleotides were performed to assess whether the DNA biosensor responded selectively, via hybridization, to the target. The variation in redox current with the different concentrations of target DNA was linear in the range of 1–20 ppm. Various properties and characteristics of this sensor were also described in this report. A novel approaches to construct an electrochemical biosensor consisting of the probe ssDNA to hybridize with crude DNA fragments from real samples was successfully applied in the study of biological microorganisms.
format Article
author Siddiquee, Shafiquzzaman
Yusof, Nor Azah
Salleh, Abu Bakar
Tan, Soon Guan
Abu Bakar, Fatimah
Lee, Yook Heng
spellingShingle Siddiquee, Shafiquzzaman
Yusof, Nor Azah
Salleh, Abu Bakar
Tan, Soon Guan
Abu Bakar, Fatimah
Lee, Yook Heng
DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
author_facet Siddiquee, Shafiquzzaman
Yusof, Nor Azah
Salleh, Abu Bakar
Tan, Soon Guan
Abu Bakar, Fatimah
Lee, Yook Heng
author_sort Siddiquee, Shafiquzzaman
title DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
title_short DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
title_full DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
title_fullStr DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
title_full_unstemmed DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
title_sort dna hybridization based on trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode.
publisher Elsevier
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/14121/
http://www.elsevier.com
_version_ 1643825536701562880
score 13.211869

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