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Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.

An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR...

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Main Authors: Ooi, Chien Wei, Tey, Beng Ti, Hii, Siew Ling, Ariff, Arbakariya, Wu, Ho Shing, Lan, John Chi Wei, Juang, RueyShin, Mustapa Kamal, Siti Mazlina, Chuan, Ling Tau
Format: Article
Language:English
Published: Springer 2009
Online Access:http://psasir.upm.edu.my/id/eprint/14082/
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spelling my.upm.eprints.140822013-11-28T02:23:35Z http://psasir.upm.edu.my/id/eprint/14082/ Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Ariff, Arbakariya Wu, Ho Shing Lan, John Chi Wei Juang, RueyShin Mustapa Kamal, Siti Mazlina Chuan, Ling Tau An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts.Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study. Springer 2009 Article PeerReviewed Ooi, Chien Wei and Tey, Beng Ti and Hii, Siew Ling and Ariff, Arbakariya and Wu, Ho Shing and Lan, John Chi Wei and Juang, RueyShin and Mustapa Kamal, Siti Mazlina and Chuan, Ling Tau (2009) Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. Biotechnology and Bioprocess Engineering, 14 (6). pp. 811-818. ISSN 1226-8372 10.1007/s12257-008-0306-y English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An aqueous two-phase purification process was employed for the recovery of Burkholderia Pseudomallei lipase from fermentation broth. The partition behavior of Burkholderia Pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts.Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study.
format Article
author Ooi, Chien Wei
Tey, Beng Ti
Hii, Siew Ling
Ariff, Arbakariya
Wu, Ho Shing
Lan, John Chi Wei
Juang, RueyShin
Mustapa Kamal, Siti Mazlina
Chuan, Ling Tau
spellingShingle Ooi, Chien Wei
Tey, Beng Ti
Hii, Siew Ling
Ariff, Arbakariya
Wu, Ho Shing
Lan, John Chi Wei
Juang, RueyShin
Mustapa Kamal, Siti Mazlina
Chuan, Ling Tau
Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
author_facet Ooi, Chien Wei
Tey, Beng Ti
Hii, Siew Ling
Ariff, Arbakariya
Wu, Ho Shing
Lan, John Chi Wei
Juang, RueyShin
Mustapa Kamal, Siti Mazlina
Chuan, Ling Tau
author_sort Ooi, Chien Wei
title Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
title_short Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
title_full Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
title_fullStr Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
title_full_unstemmed Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
title_sort direct purification of burkholderia pseudomallei lipase from fermentation broth using aqueous two-phase systems.
publisher Springer
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/14082/
_version_ 1643825525131575296
score 13.211869

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