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Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples

: The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and se...

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Main Authors: Cheah, Yoke Kqueen, Chong, Yee Tyan, Khoo, Siew Ping, Radu, Son
Format: Article
Language:English
English
English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2011
Online Access:http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf
http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf
http://psasir.upm.edu.my/id/eprint/14031/
http://www.ifrj.upm.edu.my/volume-18-2011.html
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spelling my.upm.eprints.140312015-10-06T01:49:38Z http://psasir.upm.edu.my/id/eprint/14031/ Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples Cheah, Yoke Kqueen Chong, Yee Tyan Khoo, Siew Ping Radu, Son : The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and secondly to obtain purified nucleic acids using CTAB DNA extraction method for conducting a GM specific analysis on various types of food samples. The multiplex PCR was optimized to improve PCR performance and to minimize failure. Out of 60 samples, 42 (70.0%) were found containing Cry1Ab or EPSPS genes, consisting of 11.9% of Roundup Ready Soya positive samples and 88.1% of Bt 176 Maize positive samples. Besides, 71.7% samples yielded DNA concentration above 50 ng/µl; 66.7% samples were in the DNA purity range of 1.6 to 2.0 and 85.0% of the samples were amplifiable for the endogenous gene screening. The CTAB DNA extraction method is effective for the DNA extraction from animal feeds, raw materials and processed foods. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf Cheah, Yoke Kqueen and Chong, Yee Tyan and Khoo, Siew Ping and Radu, Son (2011) Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples. International Food Research Journal, 18 (2). pp. 515-522. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
English
description : The incidence of GMO is increasing worldwide therefore development of a reliable yet cost and time saving analytical method to detect GMO is important. This study aimed to develop a multiplex-PCR for GMO detection targeting Cry1Ab and EPSPS genes in soy and maize samples simultaneously, and secondly to obtain purified nucleic acids using CTAB DNA extraction method for conducting a GM specific analysis on various types of food samples. The multiplex PCR was optimized to improve PCR performance and to minimize failure. Out of 60 samples, 42 (70.0%) were found containing Cry1Ab or EPSPS genes, consisting of 11.9% of Roundup Ready Soya positive samples and 88.1% of Bt 176 Maize positive samples. Besides, 71.7% samples yielded DNA concentration above 50 ng/µl; 66.7% samples were in the DNA purity range of 1.6 to 2.0 and 85.0% of the samples were amplifiable for the endogenous gene screening. The CTAB DNA extraction method is effective for the DNA extraction from animal feeds, raw materials and processed foods.
format Article
author Cheah, Yoke Kqueen
Chong, Yee Tyan
Khoo, Siew Ping
Radu, Son
spellingShingle Cheah, Yoke Kqueen
Chong, Yee Tyan
Khoo, Siew Ping
Radu, Son
Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
author_facet Cheah, Yoke Kqueen
Chong, Yee Tyan
Khoo, Siew Ping
Radu, Son
author_sort Cheah, Yoke Kqueen
title Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
title_short Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
title_full Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
title_fullStr Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
title_full_unstemmed Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
title_sort development of multiplex-pcr for genetically modified organism (gmo) detection targeting epsps and cry1ab genes in soy and maize samples
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/14031/1/14031.pdf
http://psasir.upm.edu.my/id/eprint/14031/7/Development%20of%20multiplex.pdf
http://psasir.upm.edu.my/id/eprint/14031/
http://www.ifrj.upm.edu.my/volume-18-2011.html
_version_ 1643825509377769472
score 13.211869

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