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Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.

Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diver...

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Main Authors: Abdullah Al-Haj, Nagi Ahmed, Shamsudin, Mariana Nor, Abdul Rahim, Raha, Ishak, Zamri
Format: Article
Language:English
English
Published: Medwell Journals 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13994/1/Development%20of%20a%20PCR%20primer%20and%20a%20marker%20band%20for%20detection%20of%20E.pdf
http://psasir.upm.edu.my/id/eprint/13994/
http://medwelljournals.com/journalhome.php?jid=1815-8846
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spelling my.upm.eprints.139942015-12-03T02:58:25Z http://psasir.upm.edu.my/id/eprint/13994/ Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called secD was designed to be used as PCR primer. secD primer pairs was highly specific to detect all isolates including E. coli O157: H7. Medwell Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13994/1/Development%20of%20a%20PCR%20primer%20and%20a%20marker%20band%20for%20detection%20of%20E.pdf Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set. Research Journal of Biological Sciences, 3 (11). pp. 1327-1332. ISSN 1815-8846; ESSN: 1993-6087 http://medwelljournals.com/journalhome.php?jid=1815-8846 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called secD was designed to be used as PCR primer. secD primer pairs was highly specific to detect all isolates including E. coli O157: H7.
format Article
author Abdullah Al-Haj, Nagi Ahmed
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Ishak, Zamri
spellingShingle Abdullah Al-Haj, Nagi Ahmed
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Ishak, Zamri
Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
author_facet Abdullah Al-Haj, Nagi Ahmed
Shamsudin, Mariana Nor
Abdul Rahim, Raha
Ishak, Zamri
author_sort Abdullah Al-Haj, Nagi Ahmed
title Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
title_short Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
title_full Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
title_fullStr Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
title_full_unstemmed Development of a PCR primer and a marker band for detection of E. coli from various sources based on arbitrary primer set.
title_sort development of a pcr primer and a marker band for detection of e. coli from various sources based on arbitrary primer set.
publisher Medwell Journals
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/13994/1/Development%20of%20a%20PCR%20primer%20and%20a%20marker%20band%20for%20detection%20of%20E.pdf
http://psasir.upm.edu.my/id/eprint/13994/
http://medwelljournals.com/journalhome.php?jid=1815-8846
_version_ 1643825498715848704
score 13.211869

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