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Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.

Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazol...

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Main Authors: Abdul, Ahmad Bustamam, Abdelwahab, Siddig Ibrahim, Jalinas, Johari, Al-Zubairi, Adel Sharaf, Taha, Manal Mohamed Elhassan
Format: Article
Language:English
English
Published: Lippincott, Williams & Wilkins 2009
Online Access:http://psasir.upm.edu.my/id/eprint/13631/1/Combination%20of%20zerumbone%20and%20cisplatin%20to%20treat%20cervical%20intraepithelial%20neoplasia%20in%20female%20BALB.pdf
http://psasir.upm.edu.my/id/eprint/13631/
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spelling my.upm.eprints.136312015-11-13T03:45:10Z http://psasir.upm.edu.my/id/eprint/13631/ Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. Abdul, Ahmad Bustamam Abdelwahab, Siddig Ibrahim Jalinas, Johari Al-Zubairi, Adel Sharaf Taha, Manal Mohamed Elhassan Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferating cellular nuclear antigen were used to study the antitumor effect of ZER.Prenatally exposed female BALB/c micewere used as a model. The progenies with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER,and a combination of both compounds. All treated and untreated mice were humanely killed, and serum and cervix were obtained for interleukin 6 analysis and histopathologic studies using hematoxylin and eosin staining, respectively. Zerumbone has revealed an antitumor effect on human cervical cancer cells and downregulates immunoexpression of proliferating cellular nuclear antigen (P < 0.05). In vivo study indicates that ZER at 16 mg/kg and CIS at 10 mg/kg have a regressing effect on CIN. The combination of ZER and CIS also showed similar effectiveness in regressing CIN. Our results indicate that the combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution (P < 0.05). The effectiveness of combining ZER and CIS could be further explored as a new therapeutic intervention of early precancerous stages of carcinogenesis before the invasive stage begins. Lippincott, Williams & Wilkins 2009-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13631/1/Combination%20of%20zerumbone%20and%20cisplatin%20to%20treat%20cervical%20intraepithelial%20neoplasia%20in%20female%20BALB.pdf Abdul, Ahmad Bustamam and Abdelwahab, Siddig Ibrahim and Jalinas, Johari and Al-Zubairi, Adel Sharaf and Taha, Manal Mohamed Elhassan (2009) Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice. International Journal of Gynecological Cancer, 19 (6). pp. 1004-1010. ISSN 1048-891X, ESSN: 1525-1438 10.1111/IGC.0b013e3181a83b51 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferating cellular nuclear antigen were used to study the antitumor effect of ZER.Prenatally exposed female BALB/c micewere used as a model. The progenies with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER,and a combination of both compounds. All treated and untreated mice were humanely killed, and serum and cervix were obtained for interleukin 6 analysis and histopathologic studies using hematoxylin and eosin staining, respectively. Zerumbone has revealed an antitumor effect on human cervical cancer cells and downregulates immunoexpression of proliferating cellular nuclear antigen (P < 0.05). In vivo study indicates that ZER at 16 mg/kg and CIS at 10 mg/kg have a regressing effect on CIN. The combination of ZER and CIS also showed similar effectiveness in regressing CIN. Our results indicate that the combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution (P < 0.05). The effectiveness of combining ZER and CIS could be further explored as a new therapeutic intervention of early precancerous stages of carcinogenesis before the invasive stage begins.
format Article
author Abdul, Ahmad Bustamam
Abdelwahab, Siddig Ibrahim
Jalinas, Johari
Al-Zubairi, Adel Sharaf
Taha, Manal Mohamed Elhassan
spellingShingle Abdul, Ahmad Bustamam
Abdelwahab, Siddig Ibrahim
Jalinas, Johari
Al-Zubairi, Adel Sharaf
Taha, Manal Mohamed Elhassan
Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
author_facet Abdul, Ahmad Bustamam
Abdelwahab, Siddig Ibrahim
Jalinas, Johari
Al-Zubairi, Adel Sharaf
Taha, Manal Mohamed Elhassan
author_sort Abdul, Ahmad Bustamam
title Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
title_short Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
title_full Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
title_fullStr Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
title_full_unstemmed Combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female BALB/c mice.
title_sort combination of zerumbone and cisplatin to treat cervical intraepithelial neoplasia in female balb/c mice.
publisher Lippincott, Williams & Wilkins
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/13631/1/Combination%20of%20zerumbone%20and%20cisplatin%20to%20treat%20cervical%20intraepithelial%20neoplasia%20in%20female%20BALB.pdf
http://psasir.upm.edu.my/id/eprint/13631/
_version_ 1643825389506658304
score 13.211869

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