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Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions

The effect of mild hypothermic (32 degrees C) conditions on cell growth, cell-cycle progress, and antibody production of hybridoma C2E7 cells was investigated in the present study. The growth of hybridoma cells was slower during the mild hypothermic condition compared to that at 37 degrees C; this l...

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Main Authors: Chong, Sun Li, Mou, Duen Gang, Ali, Abdul Manaf, Lim, Saw Hoon, Tey, Beng Ti
Format: Article
Language:English
Published: Mary Ann Liebert 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13505/1/13505.pdf
http://psasir.upm.edu.my/id/eprint/13505/
http://online.liebertpub.com/doi/abs/10.1089/hyb.2007.0548
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spelling my.upm.eprints.135052016-04-21T07:41:43Z http://psasir.upm.edu.my/id/eprint/13505/ Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions Chong, Sun Li Mou, Duen Gang Ali, Abdul Manaf Lim, Saw Hoon Tey, Beng Ti The effect of mild hypothermic (32 degrees C) conditions on cell growth, cell-cycle progress, and antibody production of hybridoma C2E7 cells was investigated in the present study. The growth of hybridoma cells was slower during the mild hypothermic condition compared to that at 37 degrees C; this led to about 10% decrease in maximum viable cell density and volumetric antibody productivity. However, under mild hypothermic growth conditions, the culture viability was substantially improved and the specific antibody productivity was enhanced compared to that at 37 degrees C. The average specific productivity for the entire batch culture at 32 degrees C is about 5% higher than that at 37 degrees C. Cell-cycle analysis data showed that there was no growth arrestment during the mild hypothermic growth of hybridoma cells. The G1-phase cells were increased, while the S-phase cells were decreased gradually as the culture time progressed. Further analysis showed that the specific antibody productivity of hybridoma cells was correlated to the fraction of S-phase cells. Mary Ann Liebert 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13505/1/13505.pdf Chong, Sun Li and Mou, Duen Gang and Ali, Abdul Manaf and Lim, Saw Hoon and Tey, Beng Ti (2008) Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions. Hybridoma, 27 (2). pp. 107-111. ISSN 1554-0014 http://online.liebertpub.com/doi/abs/10.1089/hyb.2007.0548 10.1089/hyb.2007.0548
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effect of mild hypothermic (32 degrees C) conditions on cell growth, cell-cycle progress, and antibody production of hybridoma C2E7 cells was investigated in the present study. The growth of hybridoma cells was slower during the mild hypothermic condition compared to that at 37 degrees C; this led to about 10% decrease in maximum viable cell density and volumetric antibody productivity. However, under mild hypothermic growth conditions, the culture viability was substantially improved and the specific antibody productivity was enhanced compared to that at 37 degrees C. The average specific productivity for the entire batch culture at 32 degrees C is about 5% higher than that at 37 degrees C. Cell-cycle analysis data showed that there was no growth arrestment during the mild hypothermic growth of hybridoma cells. The G1-phase cells were increased, while the S-phase cells were decreased gradually as the culture time progressed. Further analysis showed that the specific antibody productivity of hybridoma cells was correlated to the fraction of S-phase cells.
format Article
author Chong, Sun Li
Mou, Duen Gang
Ali, Abdul Manaf
Lim, Saw Hoon
Tey, Beng Ti
spellingShingle Chong, Sun Li
Mou, Duen Gang
Ali, Abdul Manaf
Lim, Saw Hoon
Tey, Beng Ti
Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
author_facet Chong, Sun Li
Mou, Duen Gang
Ali, Abdul Manaf
Lim, Saw Hoon
Tey, Beng Ti
author_sort Chong, Sun Li
title Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
title_short Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
title_full Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
title_fullStr Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
title_full_unstemmed Cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
title_sort cell growth, cell-cycle progress, and antibody production in hybridoma cells cultivated under mild hypothermic conditions
publisher Mary Ann Liebert
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/13505/1/13505.pdf
http://psasir.upm.edu.my/id/eprint/13505/
http://online.liebertpub.com/doi/abs/10.1089/hyb.2007.0548
_version_ 1643825351126679552
score 13.211869

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