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A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards

The blood of the Painted Storks (Mycteria leucocephala) and the Milky Storks (M. cinerea) from Malaysia were collected invasively from the breeding site. The blood was dropped on to FTA® cards and stored at room temperature. DNA was isolated from the FTA® cards through a modification of the Wizard...

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Main Authors: Yoke, Elsie Sim Yee, Zahari, Zainal, Ismail, Ahmad, Yap, Chee Kong, Tan, Soon Guan
Format: Article
Language:English
Published: Elsevier GmbH - Urban und Fischer 2009
Online Access:http://psasir.upm.edu.my/id/eprint/12858/1/A%20simple%20protocol%20to%20isolate%20DNA%20from%20Malaysian%20stork%20blood%20collected%20on%20FTA%20cards.pdf
http://psasir.upm.edu.my/id/eprint/12858/
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spelling my.upm.eprints.128582015-11-04T03:05:40Z http://psasir.upm.edu.my/id/eprint/12858/ A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards Yoke, Elsie Sim Yee Zahari, Zainal Ismail, Ahmad Yap, Chee Kong Tan, Soon Guan The blood of the Painted Storks (Mycteria leucocephala) and the Milky Storks (M. cinerea) from Malaysia were collected invasively from the breeding site. The blood was dropped on to FTA® cards and stored at room temperature. DNA was isolated from the FTA® cards through a modification of the Wizard DNA Purification kit (Promega) procedure and PCR was performed with 11 pairs of microsatellite primers of the American Wood Stork (M. americana). The collection of a drop of blood onto the card is superior to the usual practice of collecting about five ml of blood into a vacuum tube as it causes fewer traumas to these sensitive birds. Moreover, this collection procedure can be adopted for use in various wild animal species which are usually found in the remote areas of Malaysia as the sample collection cards can be transported back to the laboratory at room temperature. Our procedure allows the typing of several molecular genetic markers from just a drop of blood collected in the field and stored at room temperature alleviating the need for storage in expensive deep freezers or liquid nitrogen tanks. Collection of avian samples is no easy task as the birds are free flying. Elsevier GmbH - Urban und Fischer 2009-01 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12858/1/A%20simple%20protocol%20to%20isolate%20DNA%20from%20Malaysian%20stork%20blood%20collected%20on%20FTA%20cards.pdf Yoke, Elsie Sim Yee and Zahari, Zainal and Ismail, Ahmad and Yap, Chee Kong and Tan, Soon Guan (2009) A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards. Genetik, 14 (1). pp. 20-22. ISSN 0435-284X
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The blood of the Painted Storks (Mycteria leucocephala) and the Milky Storks (M. cinerea) from Malaysia were collected invasively from the breeding site. The blood was dropped on to FTA® cards and stored at room temperature. DNA was isolated from the FTA® cards through a modification of the Wizard DNA Purification kit (Promega) procedure and PCR was performed with 11 pairs of microsatellite primers of the American Wood Stork (M. americana). The collection of a drop of blood onto the card is superior to the usual practice of collecting about five ml of blood into a vacuum tube as it causes fewer traumas to these sensitive birds. Moreover, this collection procedure can be adopted for use in various wild animal species which are usually found in the remote areas of Malaysia as the sample collection cards can be transported back to the laboratory at room temperature. Our procedure allows the typing of several molecular genetic markers from just a drop of blood collected in the field and stored at room temperature alleviating the need for storage in expensive deep freezers or liquid nitrogen tanks. Collection of avian samples is no easy task as the birds are free flying.
format Article
author Yoke, Elsie Sim Yee
Zahari, Zainal
Ismail, Ahmad
Yap, Chee Kong
Tan, Soon Guan
spellingShingle Yoke, Elsie Sim Yee
Zahari, Zainal
Ismail, Ahmad
Yap, Chee Kong
Tan, Soon Guan
A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards
author_facet Yoke, Elsie Sim Yee
Zahari, Zainal
Ismail, Ahmad
Yap, Chee Kong
Tan, Soon Guan
author_sort Yoke, Elsie Sim Yee
title A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards
title_short A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards
title_full A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards
title_fullStr A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards
title_full_unstemmed A simple protocol to isolate DNA from Malaysian stork blood collected on FTA cards
title_sort simple protocol to isolate dna from malaysian stork blood collected on fta cards
publisher Elsevier GmbH - Urban und Fischer
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/12858/1/A%20simple%20protocol%20to%20isolate%20DNA%20from%20Malaysian%20stork%20blood%20collected%20on%20FTA%20cards.pdf
http://psasir.upm.edu.my/id/eprint/12858/
_version_ 1643825155865051136
score 13.211869

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