A simple method for quantification of interferon-α2b through surface plasmon resonance technique

A rapid and efficient immunoassay method for quantification of interferon-α2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding rati...

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Main Authors: Ramanan, Ramakrishnan Nagasundara, Tau, Chuan Ling, Tey, Beng Ti, Ariff, Arbakariya
格式: Article
语言:English
出版: Academic Journals 2010
在线阅读:http://psasir.upm.edu.my/id/eprint/12856/1/12856.pdf
http://psasir.upm.edu.my/id/eprint/12856/
http://www.academicjournals.org/journal/AJB/article-abstract/31EEEA018087
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spelling my.upm.eprints.128562017-11-14T03:07:41Z http://psasir.upm.edu.my/id/eprint/12856/ A simple method for quantification of interferon-α2b through surface plasmon resonance technique Ramanan, Ramakrishnan Nagasundara Tau, Chuan Ling Tey, Beng Ti Ariff, Arbakariya A rapid and efficient immunoassay method for quantification of interferon-α2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding ratio was observed for both the ligand densities. There was no steric hindrance and loss of antibody activity even at higher ligand density (> 22,000 RU). The sensitivity of the assay was increased up to 45% with the increment in ligand density from 15,400 to 22,360 RU. The binding between interferon-α2b and anti-interferon monoclonal antibody was predominantly controlled by mass transfer rate and the relationship was found linear, ranged from 5 to 400 ng/mL. Total cycle time per analysis was less than 8 min and required only 5 μL of sample injection. Academic Journals 2010-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12856/1/12856.pdf Ramanan, Ramakrishnan Nagasundara and Tau, Chuan Ling and Tey, Beng Ti and Ariff, Arbakariya (2010) A simple method for quantification of interferon-α2b through surface plasmon resonance technique. African Journal of Biotechnology, 9 (11). art. no. 31EEEA018087. pp. 1680-1689. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/31EEEA018087
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A rapid and efficient immunoassay method for quantification of interferon-α2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding ratio was observed for both the ligand densities. There was no steric hindrance and loss of antibody activity even at higher ligand density (> 22,000 RU). The sensitivity of the assay was increased up to 45% with the increment in ligand density from 15,400 to 22,360 RU. The binding between interferon-α2b and anti-interferon monoclonal antibody was predominantly controlled by mass transfer rate and the relationship was found linear, ranged from 5 to 400 ng/mL. Total cycle time per analysis was less than 8 min and required only 5 μL of sample injection.
format Article
author Ramanan, Ramakrishnan Nagasundara
Tau, Chuan Ling
Tey, Beng Ti
Ariff, Arbakariya
spellingShingle Ramanan, Ramakrishnan Nagasundara
Tau, Chuan Ling
Tey, Beng Ti
Ariff, Arbakariya
A simple method for quantification of interferon-α2b through surface plasmon resonance technique
author_facet Ramanan, Ramakrishnan Nagasundara
Tau, Chuan Ling
Tey, Beng Ti
Ariff, Arbakariya
author_sort Ramanan, Ramakrishnan Nagasundara
title A simple method for quantification of interferon-α2b through surface plasmon resonance technique
title_short A simple method for quantification of interferon-α2b through surface plasmon resonance technique
title_full A simple method for quantification of interferon-α2b through surface plasmon resonance technique
title_fullStr A simple method for quantification of interferon-α2b through surface plasmon resonance technique
title_full_unstemmed A simple method for quantification of interferon-α2b through surface plasmon resonance technique
title_sort simple method for quantification of interferon-α2b through surface plasmon resonance technique
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/12856/1/12856.pdf
http://psasir.upm.edu.my/id/eprint/12856/
http://www.academicjournals.org/journal/AJB/article-abstract/31EEEA018087
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