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A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria

In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage...

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Main Authors: Abd. Shukor, Mohd. Yunus, Shamsuddin, Burhanuddin, Mohamad, Othman, Ithnin, Khalid, Shamaan, Nor Aripin, Syed, Mohd. Arif
Format: Article
Language:English
Published: Asian Network for Scientific Information 2008
Online Access:http://psasir.upm.edu.my/id/eprint/12722/1/A%20method%20to%20study%20the%20effects%20of%20chemical%20and%20biological%20reduction%20of%20molybdate%20to%20molybdenum%20blue%20in%20bacteria.pdf
http://psasir.upm.edu.my/id/eprint/12722/
http://scialert.net/abstract/?doi=pjbs.2008.672.675
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spelling my.upm.eprints.127222015-12-02T03:29:01Z http://psasir.upm.edu.my/id/eprint/12722/ A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria Abd. Shukor, Mohd. Yunus Shamsuddin, Burhanuddin Mohamad, Othman Ithnin, Khalid Shamaan, Nor Aripin Syed, Mohd. Arif In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage to prove that inhibition of molybdate reduction by phosphate and arsenate is at the level of phosphomolybdate and not cellular. We also prove that mercury is a physiological inhibitor to molybdate reduction. We suggest the use of this method to assess the effect of inhibitors and activators to molybdate reduction in bacteria. Asian Network for Scientific Information 2008-02-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12722/1/A%20method%20to%20study%20the%20effects%20of%20chemical%20and%20biological%20reduction%20of%20molybdate%20to%20molybdenum%20blue%20in%20bacteria.pdf Abd. Shukor, Mohd. Yunus and Shamsuddin, Burhanuddin and Mohamad, Othman and Ithnin, Khalid and Shamaan, Nor Aripin and Syed, Mohd. Arif (2008) A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria. Pakistan Journal of Biological Sciences, 11 (4). pp. 672-675. ISSN 1028-8880; ESSN: 1812-5735 http://scialert.net/abstract/?doi=pjbs.2008.672.675 10.3923/pjbs.2008.672.675
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage to prove that inhibition of molybdate reduction by phosphate and arsenate is at the level of phosphomolybdate and not cellular. We also prove that mercury is a physiological inhibitor to molybdate reduction. We suggest the use of this method to assess the effect of inhibitors and activators to molybdate reduction in bacteria.
format Article
author Abd. Shukor, Mohd. Yunus
Shamsuddin, Burhanuddin
Mohamad, Othman
Ithnin, Khalid
Shamaan, Nor Aripin
Syed, Mohd. Arif
spellingShingle Abd. Shukor, Mohd. Yunus
Shamsuddin, Burhanuddin
Mohamad, Othman
Ithnin, Khalid
Shamaan, Nor Aripin
Syed, Mohd. Arif
A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
author_facet Abd. Shukor, Mohd. Yunus
Shamsuddin, Burhanuddin
Mohamad, Othman
Ithnin, Khalid
Shamaan, Nor Aripin
Syed, Mohd. Arif
author_sort Abd. Shukor, Mohd. Yunus
title A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
title_short A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
title_full A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
title_fullStr A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
title_full_unstemmed A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
title_sort method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria
publisher Asian Network for Scientific Information
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/12722/1/A%20method%20to%20study%20the%20effects%20of%20chemical%20and%20biological%20reduction%20of%20molybdate%20to%20molybdenum%20blue%20in%20bacteria.pdf
http://psasir.upm.edu.my/id/eprint/12722/
http://scialert.net/abstract/?doi=pjbs.2008.672.675
_version_ 1643825115217002496
score 13.211869

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