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Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson

Phalaenopsis bellina (Rchb.f.) Christenson orchid species are known for their beautiful flower shape, graceful inflorescence and fragrance. Protocorm-like bodies (PLBs) of P. bellina were induced from leaf segments. The PLBs were then subjected to proliferation using ½ strength Murashige and Skoog (...

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Main Authors: Khoddamzadeh, Amir Ali, Sinniah, Uma Rani, Abdul Kadir, Mihdzar, Kadzimin, Saleh, Mahmood, Maziah, Subramaniam, Sreeramanan
Format: Article
Language:English
Published: Academic Journals 2010
Online Access:http://psasir.upm.edu.my/id/eprint/12385/1/Detection_of_Somaclonal_Variation.pdf
http://psasir.upm.edu.my/id/eprint/12385/
http://www.academicjournals.org/journal/AJB/article-abstract/DA2DBE819146
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spelling my.upm.eprints.123852017-11-15T08:11:45Z http://psasir.upm.edu.my/id/eprint/12385/ Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson Khoddamzadeh, Amir Ali Sinniah, Uma Rani Abdul Kadir, Mihdzar Kadzimin, Saleh Mahmood, Maziah Subramaniam, Sreeramanan Phalaenopsis bellina (Rchb.f.) Christenson orchid species are known for their beautiful flower shape, graceful inflorescence and fragrance. Protocorm-like bodies (PLBs) of P. bellina were induced from leaf segments. The PLBs were then subjected to proliferation using ½ strength Murashige and Skoog (MS) media with two subcultures at three months intervals. Twelve decamer random amplified polymorphic DNA (RAPD) primers were used to study somaclonal variation among the mother plant, the initially induced PLBs and proliferated PLBs after 3 and 6 months in culture. Eight out of twelve primers produced 172 bands with 18 polymorphic bands in all the treatments. The amplified products varied between 125 to 8000 bp. Among the primers used, P 16 produced the highest number of bands (29), while primer OPU 10 produced the lowest number (15). The range of similarity coefficient was from 0.83 to 1.0 among the different sub-cultures and mother plant (MP). It was found that minimal or no changes occurred between the MP and the PLBs produced after 3 months of induction. The induced PLBs were then subcultured for six months for proliferation and this resulted in about 17% dissimilarity with MP. It is reported that micropropagation of P. bellina can be carried out successfully using ½ strength MS media for 6 months but further proliferation may result in somaclonal variation which might change the prolific characteristic of this orchids. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12385/1/Detection_of_Somaclonal_Variation.pdf Khoddamzadeh, Amir Ali and Sinniah, Uma Rani and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Mahmood, Maziah and Subramaniam, Sreeramanan (2010) Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson. African Journal of Biotechnology, 9 (40). art. no. DA2DBE819146. pp. 6632-6639. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/DA2DBE819146
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Phalaenopsis bellina (Rchb.f.) Christenson orchid species are known for their beautiful flower shape, graceful inflorescence and fragrance. Protocorm-like bodies (PLBs) of P. bellina were induced from leaf segments. The PLBs were then subjected to proliferation using ½ strength Murashige and Skoog (MS) media with two subcultures at three months intervals. Twelve decamer random amplified polymorphic DNA (RAPD) primers were used to study somaclonal variation among the mother plant, the initially induced PLBs and proliferated PLBs after 3 and 6 months in culture. Eight out of twelve primers produced 172 bands with 18 polymorphic bands in all the treatments. The amplified products varied between 125 to 8000 bp. Among the primers used, P 16 produced the highest number of bands (29), while primer OPU 10 produced the lowest number (15). The range of similarity coefficient was from 0.83 to 1.0 among the different sub-cultures and mother plant (MP). It was found that minimal or no changes occurred between the MP and the PLBs produced after 3 months of induction. The induced PLBs were then subcultured for six months for proliferation and this resulted in about 17% dissimilarity with MP. It is reported that micropropagation of P. bellina can be carried out successfully using ½ strength MS media for 6 months but further proliferation may result in somaclonal variation which might change the prolific characteristic of this orchids.
format Article
author Khoddamzadeh, Amir Ali
Sinniah, Uma Rani
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Mahmood, Maziah
Subramaniam, Sreeramanan
spellingShingle Khoddamzadeh, Amir Ali
Sinniah, Uma Rani
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Mahmood, Maziah
Subramaniam, Sreeramanan
Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson
author_facet Khoddamzadeh, Amir Ali
Sinniah, Uma Rani
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Mahmood, Maziah
Subramaniam, Sreeramanan
author_sort Khoddamzadeh, Amir Ali
title Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson
title_short Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson
title_full Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson
title_fullStr Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson
title_full_unstemmed Detection of somaclonal variation by random amplified polymorphic DNA analysis during micropropagation of Phalaenopsis bellina (Rchb.f.) Christenson
title_sort detection of somaclonal variation by random amplified polymorphic dna analysis during micropropagation of phalaenopsis bellina (rchb.f.) christenson
publisher Academic Journals
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/12385/1/Detection_of_Somaclonal_Variation.pdf
http://psasir.upm.edu.my/id/eprint/12385/
http://www.academicjournals.org/journal/AJB/article-abstract/DA2DBE819146
_version_ 1643825025951727616
score 13.211869

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