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Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat

A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to h...

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Main Author: Chandrasiri, Alawattage Don Nimal
Format: Thesis
Language:English
English
Published: 1997
Online Access:http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf
http://psasir.upm.edu.my/id/eprint/12327/
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spelling my.upm.eprints.123272011-09-12T01:34:30Z http://psasir.upm.edu.my/id/eprint/12327/ Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat Chandrasiri, Alawattage Don Nimal A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to heparin could be used for sperm capacitation. The acrosome reaction was tested by a dual staining technique (Experiment 1 ) and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the 2-cell stage (Experiment 3). Freshly ejaculated semen from two fertile bucks were used for sperm capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15 min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm treated with heparin and S-ACT - 1 showed similar staining characteristics. 1997-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf Chandrasiri, Alawattage Don Nimal (1997) Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat. PhD thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to heparin could be used for sperm capacitation. The acrosome reaction was tested by a dual staining technique (Experiment 1 ) and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the 2-cell stage (Experiment 3). Freshly ejaculated semen from two fertile bucks were used for sperm capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15 min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm treated with heparin and S-ACT - 1 showed similar staining characteristics.
format Thesis
author Chandrasiri, Alawattage Don Nimal
spellingShingle Chandrasiri, Alawattage Don Nimal
Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
author_facet Chandrasiri, Alawattage Don Nimal
author_sort Chandrasiri, Alawattage Don Nimal
title Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
title_short Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
title_full Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
title_fullStr Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
title_full_unstemmed Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
title_sort extract of gelidiella acerosa (s-act-1) as a capacitation agent for in vitro fertilisation in goat
publishDate 1997
url http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf
http://psasir.upm.edu.my/id/eprint/12327/
_version_ 1643825009165074432
score 13.211869

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