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Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column

The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w...

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Main Authors: Luechau, Frank, Tau, Chuan Ling, Lyddiatt, Andrew
Format: Article
Language:English
English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/12125/1/Physical%20characterisations%20of%20a%20single.pdf
http://psasir.upm.edu.my/id/eprint/12125/
http://dx.doi.org/10.1016/j.bej.2010.02.010
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spelling my.upm.eprints.121252015-10-05T03:12:11Z http://psasir.upm.edu.my/id/eprint/12125/ Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w) di-potassium hydrogen phosphate (designated as 12/12) and 12% (w/w) PEG 1450 and 11% (w/w) di-potassium hydrogen phosphate (designated as 12/11) were chosen in this study. The hold-up D increased with increasing impeller speeds and mobile phase flow rates. Phase separation for the 12/11 system was slower than that for the 12/12 system, which resulted in higher dispersed phase hold-up values for the 12/11 system. For 12/12 system, mass transfer of plasmid DNA (pDNA) from the dispersed mobile phase to the stationary phase increased rapidly with increasing impeller speeds of 130, 160 and 200 rpm which was reflected in the decreased values for CT/CTo. The degree of back-mixing quantified by the axial dispersion coefficient Dax was estimated to be 2.7 × 10−6 m2 s−1. 2010-07-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12125/1/Physical%20characterisations%20of%20a%20single.pdf Luechau, Frank and Tau, Chuan Ling and Lyddiatt, Andrew (2010) Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column. Biochemical Engineering Journal, 50 (3). pp. 90-98. ISSN 1369-703X http://dx.doi.org/10.1016/j.bej.2010.02.010 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The main parameters which influence the behaviour of phase separation in a single-stage Kühni-type aqueous two-phase extraction column containing polyethylene (PEG) and di-potassium hydrogen phosphate were characterised. Two aqueous two-phase system (ATPS) composed of 12% (w/w) PEG 1450 and 12% (w/w) di-potassium hydrogen phosphate (designated as 12/12) and 12% (w/w) PEG 1450 and 11% (w/w) di-potassium hydrogen phosphate (designated as 12/11) were chosen in this study. The hold-up D increased with increasing impeller speeds and mobile phase flow rates. Phase separation for the 12/11 system was slower than that for the 12/12 system, which resulted in higher dispersed phase hold-up values for the 12/11 system. For 12/12 system, mass transfer of plasmid DNA (pDNA) from the dispersed mobile phase to the stationary phase increased rapidly with increasing impeller speeds of 130, 160 and 200 rpm which was reflected in the decreased values for CT/CTo. The degree of back-mixing quantified by the axial dispersion coefficient Dax was estimated to be 2.7 × 10−6 m2 s−1.
format Article
author Luechau, Frank
Tau, Chuan Ling
Lyddiatt, Andrew
spellingShingle Luechau, Frank
Tau, Chuan Ling
Lyddiatt, Andrew
Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
author_facet Luechau, Frank
Tau, Chuan Ling
Lyddiatt, Andrew
author_sort Luechau, Frank
title Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
title_short Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
title_full Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
title_fullStr Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
title_full_unstemmed Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
title_sort physical characterisations of a single-stage kühni-type aqueous two-phase extraction column
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/12125/1/Physical%20characterisations%20of%20a%20single.pdf
http://psasir.upm.edu.my/id/eprint/12125/
http://dx.doi.org/10.1016/j.bej.2010.02.010
_version_ 1643824950652436480
score 13.211869

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