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Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers

Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein compone...

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Main Author: Teo, Chee How
Format: Thesis
Language:English
English
Published: 2002
Subjects:
Bananas - Malaysia
Food technology
Online Access:http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf
http://psasir.upm.edu.my/id/eprint/11859/
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spelling my.upm.eprints.118592024-06-27T01:30:55Z http://psasir.upm.edu.my/id/eprint/11859/ Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers Teo, Chee How Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein components, which are required for their movement in the genome. We have exploited the repetitive, dispersed nature of many LTR-containing retrotransposon families for the visualization of genomic polymorphism (genome constitution) and the visualization of varieties polymorphism (varieties classification). The IRAP-PCR (Inter- Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are generated by the proximity of two LTRs or reverse transcriptase CRT) genes using outward-facing primers annealing to LTR target sequence. This method could distinguish between banana genome constitution (A and B genome) and varieties. This method can track new insertion of retrotransposon in banana tissue culture materials. The evolution of Malaysian bananas can also be tracked using this phylogenetic analysis. Since the method is PCR-based, it may allow the detection and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material. This method is applicable to other plants with dispersed families of LTR retrotransposons where either the LTR or nearby internal sequences are known. 2002-04 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf Teo, Chee How (2002) Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers. Masters thesis, Universiti Putra Malaysia. Bananas - Malaysia Food technology English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
topic Bananas - Malaysia
Food technology
spellingShingle Bananas - Malaysia
Food technology
Teo, Chee How
Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
description Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein components, which are required for their movement in the genome. We have exploited the repetitive, dispersed nature of many LTR-containing retrotransposon families for the visualization of genomic polymorphism (genome constitution) and the visualization of varieties polymorphism (varieties classification). The IRAP-PCR (Inter- Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are generated by the proximity of two LTRs or reverse transcriptase CRT) genes using outward-facing primers annealing to LTR target sequence. This method could distinguish between banana genome constitution (A and B genome) and varieties. This method can track new insertion of retrotransposon in banana tissue culture materials. The evolution of Malaysian bananas can also be tracked using this phylogenetic analysis. Since the method is PCR-based, it may allow the detection and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material. This method is applicable to other plants with dispersed families of LTR retrotransposons where either the LTR or nearby internal sequences are known.
format Thesis
author Teo, Chee How
author_facet Teo, Chee How
author_sort Teo, Chee How
title Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_short Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_full Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_fullStr Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_full_unstemmed Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_sort classification of the malaysian banana varieties using retrotransposon markers
publishDate 2002
url http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf
http://psasir.upm.edu.my/id/eprint/11859/
_version_ 1803336771275063296
score 13.211869

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