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Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link

One of the most promising processes using lipase which will offer great potential application especially in term of cost reduction, is the use of mycelium-bound lipase (naturally immobilized lipase). However, there has been little work reported on the technology using mycelium-bound lipase (natur...

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Main Author: Long, Kamariah
Format: Thesis
Language:English
English
Published: 1997
Online Access:http://psasir.upm.edu.my/id/eprint/11837/1/FSMB_1997_13_A.pdf
http://psasir.upm.edu.my/id/eprint/11837/
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spelling my.upm.eprints.118372012-10-29T07:50:47Z http://psasir.upm.edu.my/id/eprint/11837/ Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link Long, Kamariah One of the most promising processes using lipase which will offer great potential application especially in term of cost reduction, is the use of mycelium-bound lipase (naturally immobilized lipase). However, there has been little work reported on the technology using mycelium-bound lipase (naturally immobilize) and work using it, is limited to a few microorganisms. This study was conducted with the aims to identifying a new source of mycelium-bound lipase, and consequently to study it's production, properties and applications.Aspergillus flavus, isolated from copra meal, produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6.5 and with 2% (w/v) each of com oil and yeast extract as carbon and organic nitrogen sources, respectively. Yeast extract affects not only the production of lipase but also the secretion of proteases into the culture medium. The presence of EDTA improved the productivity of the mycelium-bound lipase by 26% although growth of A. flavus was inhibited by 11%. The addition of Tween 80, into culture medium, decreased the activity of mycelium-bound lipase. 1997-09 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11837/1/FSMB_1997_13_A.pdf Long, Kamariah (1997) Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link. PhD thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description One of the most promising processes using lipase which will offer great potential application especially in term of cost reduction, is the use of mycelium-bound lipase (naturally immobilized lipase). However, there has been little work reported on the technology using mycelium-bound lipase (naturally immobilize) and work using it, is limited to a few microorganisms. This study was conducted with the aims to identifying a new source of mycelium-bound lipase, and consequently to study it's production, properties and applications.Aspergillus flavus, isolated from copra meal, produces a lipase (EC 3.1.1.3) which is partly bound to the mycelium. The production of the mycelium-bound lipase is concomitant with growth, and declines when growth ceases. Maximum productivity of the enzyme is obtained when the culture is incubated at 30°C, an initial culture pH of 6.5 and with 2% (w/v) each of com oil and yeast extract as carbon and organic nitrogen sources, respectively. Yeast extract affects not only the production of lipase but also the secretion of proteases into the culture medium. The presence of EDTA improved the productivity of the mycelium-bound lipase by 26% although growth of A. flavus was inhibited by 11%. The addition of Tween 80, into culture medium, decreased the activity of mycelium-bound lipase.
format Thesis
author Long, Kamariah
spellingShingle Long, Kamariah
Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
author_facet Long, Kamariah
author_sort Long, Kamariah
title Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_short Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_full Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_fullStr Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_full_unstemmed Production, Properties And Applications Of Mycelium-Bound Lipase Of A Locally Isolated Strain Of Aspergillus Flavus Link
title_sort production, properties and applications of mycelium-bound lipase of a locally isolated strain of aspergillus flavus link
publishDate 1997
url http://psasir.upm.edu.my/id/eprint/11837/1/FSMB_1997_13_A.pdf
http://psasir.upm.edu.my/id/eprint/11837/
_version_ 1643824879907110912
score 13.211869

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