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Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species

Three virus particles were isolated in vitro and tentatively classified into three groupings , namely Herpesviridae, Birnaviridae and Iridoviridae . Virus groupings were based on the ultrastructural morphology and cytopathology observed . Distinct virus-like cytopathic effects were noted on fou...

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Main Author: Juan Deanon, Albaladejo
Format: Thesis
Language:English
English
Published: 1994
Online Access:http://psasir.upm.edu.my/id/eprint/11797/1/FPSS_1994_4_A.pdf
http://psasir.upm.edu.my/id/eprint/11797/
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spelling my.upm.eprints.117972011-08-10T07:12:51Z http://psasir.upm.edu.my/id/eprint/11797/ Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species Juan Deanon, Albaladejo Three virus particles were isolated in vitro and tentatively classified into three groupings , namely Herpesviridae, Birnaviridae and Iridoviridae . Virus groupings were based on the ultrastructural morphology and cytopathology observed . Distinct virus-like cytopathic effects were noted on four established fish cell lines viz . CHSE-214 , GCK-84 , EPC and TmB , utilized in the study . The virus - like particles , from pelleted infected cell cultures and clarified cell culture supernatant, were visualized under transmission electron microscopy .Virus titration utilizing BB cells for the Birnavirus - like isolate achieved a mean titre of 7.40 X 105 TC ID50/ml. Relatively high virus titres were also recorded from the other two isolants , with a mean virus titres of 7.16 X 105 TC ID50/ml for Herpesvirus - like and 6.4 6 'x 105 TCID50/ml for Iridovirus-like . 1994-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11797/1/FPSS_1994_4_A.pdf Juan Deanon, Albaladejo (1994) Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species. Masters thesis, Universiti Pertanian Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Three virus particles were isolated in vitro and tentatively classified into three groupings , namely Herpesviridae, Birnaviridae and Iridoviridae . Virus groupings were based on the ultrastructural morphology and cytopathology observed . Distinct virus-like cytopathic effects were noted on four established fish cell lines viz . CHSE-214 , GCK-84 , EPC and TmB , utilized in the study . The virus - like particles , from pelleted infected cell cultures and clarified cell culture supernatant, were visualized under transmission electron microscopy .Virus titration utilizing BB cells for the Birnavirus - like isolate achieved a mean titre of 7.40 X 105 TC ID50/ml. Relatively high virus titres were also recorded from the other two isolants , with a mean virus titres of 7.16 X 105 TC ID50/ml for Herpesvirus - like and 6.4 6 'x 105 TCID50/ml for Iridovirus-like .
format Thesis
author Juan Deanon, Albaladejo
spellingShingle Juan Deanon, Albaladejo
Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
author_facet Juan Deanon, Albaladejo
author_sort Juan Deanon, Albaladejo
title Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
title_short Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
title_full Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
title_fullStr Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
title_full_unstemmed Virus Screening Of Cultured Carp And Tilapia Species And The Virus Sensitivity Of Cell Cultures From Indigenous Fish Species
title_sort virus screening of cultured carp and tilapia species and the virus sensitivity of cell cultures from indigenous fish species
publishDate 1994
url http://psasir.upm.edu.my/id/eprint/11797/1/FPSS_1994_4_A.pdf
http://psasir.upm.edu.my/id/eprint/11797/
_version_ 1643824869771575296
score 13.211869

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