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Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant

Foot-and-mouth disease (FMD) is a major infectious disease caused by the foot-and-mouth disease virus (FMDV) that affects the domestic and foreign trade in livestock and animal products, resulting in high economic losses and social consequences. Current control of FMD includes prevention by vacci...

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Main Authors: Othman, Siti Sarah, Abd Halin, Farah Najwa, Ismail, Saila, Zakaria, Zunita
Format: Conference or Workshop Item
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2024
Online Access:http://psasir.upm.edu.my/id/eprint/115492/1/115492.pdf
http://psasir.upm.edu.my/id/eprint/115492/
https://www.msmbb.my/images/publication/volume_32/issue_2/APJMBB_Vol_32_Issue_2_Supp.pdf , https://www.scopus.com/sourceid/14964?origin=resultslist
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spelling my.upm.eprints.1154922025-03-06T01:40:34Z http://psasir.upm.edu.my/id/eprint/115492/ Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant Othman, Siti Sarah Abd Halin, Farah Najwa Ismail, Saila Zakaria, Zunita Foot-and-mouth disease (FMD) is a major infectious disease caused by the foot-and-mouth disease virus (FMDV) that affects the domestic and foreign trade in livestock and animal products, resulting in high economic losses and social consequences. Current control of FMD includes prevention by vaccination with inactivated vaccines. However, various challenges arise, including difficulty handling multiple serotypes, due to the vaccines specific for a single serotype and providing short-term immunity. Previous studies have utilised capsid protein, P1 which encodes four structural proteins (VP4, VP2, VP3, and VP1) of the FMDV to develop potential vaccines due to antigenic variation. Despite this, the antigenicity of the Malaysian FMDV capsid protein is not well studied. This study aims to characterise the Malaysian FMDV serotypes O and A capsid protein via in silico and in vitro analysis. The FMDV serotypes O and A capsid protein sequences were identified based on the FMD outbreaks in Malaysia and the antigenicity was predicted by bioinformatics approaches and computational techniques. Then, the antigenic genes were cloned into an expression vector, pET-28a and further manipulated for protein expression analysis. The capsid proteins were predicted to be antigenic by Kolaskar and Tongaonkar's semi-empirical method. The antigenic genes were successfully characterised and expressed in E. coli BL21(DE3). The results provide information on the stability of the capsid protein and the future ability to elicit immune responses. Therefore, this research can be utilized to design a novel vaccine to combat FMD in ruminants. Malaysian Society for Molecular Biology and Biotechnology 2024 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115492/1/115492.pdf Othman, Siti Sarah and Abd Halin, Farah Najwa and Ismail, Saila and Zakaria, Zunita (2024) Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant. In: 7th International Conference on Molecular Biology and Biotechnology 2024 (ICMBB2024) & 6th International Health Conference (IHEC2024), 26-27 Jun. 2024, USIM, Nilai, Malaysia. (p. 84). (Submitted) https://www.msmbb.my/images/publication/volume_32/issue_2/APJMBB_Vol_32_Issue_2_Supp.pdf , https://www.scopus.com/sourceid/14964?origin=resultslist
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Foot-and-mouth disease (FMD) is a major infectious disease caused by the foot-and-mouth disease virus (FMDV) that affects the domestic and foreign trade in livestock and animal products, resulting in high economic losses and social consequences. Current control of FMD includes prevention by vaccination with inactivated vaccines. However, various challenges arise, including difficulty handling multiple serotypes, due to the vaccines specific for a single serotype and providing short-term immunity. Previous studies have utilised capsid protein, P1 which encodes four structural proteins (VP4, VP2, VP3, and VP1) of the FMDV to develop potential vaccines due to antigenic variation. Despite this, the antigenicity of the Malaysian FMDV capsid protein is not well studied. This study aims to characterise the Malaysian FMDV serotypes O and A capsid protein via in silico and in vitro analysis. The FMDV serotypes O and A capsid protein sequences were identified based on the FMD outbreaks in Malaysia and the antigenicity was predicted by bioinformatics approaches and computational techniques. Then, the antigenic genes were cloned into an expression vector, pET-28a and further manipulated for protein expression analysis. The capsid proteins were predicted to be antigenic by Kolaskar and Tongaonkar's semi-empirical method. The antigenic genes were successfully characterised and expressed in E. coli BL21(DE3). The results provide information on the stability of the capsid protein and the future ability to elicit immune responses. Therefore, this research can be utilized to design a novel vaccine to combat FMD in ruminants.
format Conference or Workshop Item
author Othman, Siti Sarah
Abd Halin, Farah Najwa
Ismail, Saila
Zakaria, Zunita
spellingShingle Othman, Siti Sarah
Abd Halin, Farah Najwa
Ismail, Saila
Zakaria, Zunita
Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant
author_facet Othman, Siti Sarah
Abd Halin, Farah Najwa
Ismail, Saila
Zakaria, Zunita
author_sort Othman, Siti Sarah
title Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant
title_short Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant
title_full Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant
title_fullStr Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant
title_full_unstemmed Potential antigen in combating the pervasive threat of the Malaysian foot and mouth disease virus (FMDV) in ruminant
title_sort potential antigen in combating the pervasive threat of the malaysian foot and mouth disease virus (fmdv) in ruminant
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/115492/1/115492.pdf
http://psasir.upm.edu.my/id/eprint/115492/
https://www.msmbb.my/images/publication/volume_32/issue_2/APJMBB_Vol_32_Issue_2_Supp.pdf , https://www.scopus.com/sourceid/14964?origin=resultslist
_version_ 1825810762121084928
score 13.244413

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