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Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable met...

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Main Authors: Eskandari, Azadeh, Nezhad, Nima Ghahremani, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya
Format: Article
Published: Springer Science and Business Media Deutschland GmbH 2024
Online Access:http://psasir.upm.edu.my/id/eprint/115291/
https://link.springer.com/article/10.1007/s00203-024-03871-2
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spelling my.upm.eprints.1152912025-02-28T01:43:35Z http://psasir.upm.edu.my/id/eprint/115291/ Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli Eskandari, Azadeh Nezhad, Nima Ghahremani Leow, Thean Chor Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable method for biotherapeutics and food industry productions. Hence, it is crucial to analyze the limitations and advantages of microbial hosts to enhance the efficient production of recombinant proteins on a large scale. E. coli is widely used as a host for the production of recombinant proteins. Researchers have identified certain obstacles with this host, and given the growing demand for recombinant protein production, there is an immediate requirement to enhance this host. The following review discusses the elements contributing to the manifestation of recombinant protein. Subsequently, it sheds light on innovative approaches aimed at improving the expression of recombinant protein. Lastly, it delves into the obstacles and optimization methods associated with translation, mentioning both cis-optimization and trans-optimization, producing soluble recombinant protein, and engineering the metal ion transportation. In this context, a comprehensive description of the distinct features will be provided, and this knowledge could potentially enhance the expression of recombinant proteins in E. coli. Springer Science and Business Media Deutschland GmbH 2024-03-12 Article PeerReviewed Eskandari, Azadeh and Nezhad, Nima Ghahremani and Leow, Thean Chor and Abdul Rahman, Mohd Basyaruddin and Oslan, Siti Nurbaya (2024) Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli. Archives of Microbiology, 206 (4). art. no. 152. ISSN 0302-8933; eISSN: 1432-072X https://link.springer.com/article/10.1007/s00203-024-03871-2 10.1007/s00203-024-03871-2
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable method for biotherapeutics and food industry productions. Hence, it is crucial to analyze the limitations and advantages of microbial hosts to enhance the efficient production of recombinant proteins on a large scale. E. coli is widely used as a host for the production of recombinant proteins. Researchers have identified certain obstacles with this host, and given the growing demand for recombinant protein production, there is an immediate requirement to enhance this host. The following review discusses the elements contributing to the manifestation of recombinant protein. Subsequently, it sheds light on innovative approaches aimed at improving the expression of recombinant protein. Lastly, it delves into the obstacles and optimization methods associated with translation, mentioning both cis-optimization and trans-optimization, producing soluble recombinant protein, and engineering the metal ion transportation. In this context, a comprehensive description of the distinct features will be provided, and this knowledge could potentially enhance the expression of recombinant proteins in E. coli.
format Article
author Eskandari, Azadeh
Nezhad, Nima Ghahremani
Leow, Thean Chor
Abdul Rahman, Mohd Basyaruddin
Oslan, Siti Nurbaya
spellingShingle Eskandari, Azadeh
Nezhad, Nima Ghahremani
Leow, Thean Chor
Abdul Rahman, Mohd Basyaruddin
Oslan, Siti Nurbaya
Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
author_facet Eskandari, Azadeh
Nezhad, Nima Ghahremani
Leow, Thean Chor
Abdul Rahman, Mohd Basyaruddin
Oslan, Siti Nurbaya
author_sort Eskandari, Azadeh
title Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
title_short Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
title_full Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
title_fullStr Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
title_full_unstemmed Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
title_sort essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in escherichia coli
publisher Springer Science and Business Media Deutschland GmbH
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/115291/
https://link.springer.com/article/10.1007/s00203-024-03871-2
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score 13.244413

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