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Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum

A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced s...

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Main Authors: Shuhaimi, M., Ali, A.M., Saleh, N.M., Yazid, A.M.
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2001
Online Access:http://psasir.upm.edu.my/id/eprint/115211/1/115211.pdf
http://psasir.upm.edu.my/id/eprint/115211/
https://link.springer.com/article/10.1023/A:1012400518207?error=cookies_not_supported&code=b74611f1-a6e5-471f-9e24-c02e186de39a
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spelling my.upm.eprints.1152112025-02-26T03:52:29Z http://psasir.upm.edu.my/id/eprint/115211/ Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced showing a high similarity to bsh gene previously published. The resulting nucleotide sequence encodes a predicted protein of 317 amino acids, Mw = 35 kDa. Springer Science and Business Media LLC 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115211/1/115211.pdf Shuhaimi, M. and Ali, A.M. and Saleh, N.M. and Yazid, A.M. (2001) Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum. Biotechnology Letters, 23 (21). pp. 1775-1780. ISSN 0141-5492 https://link.springer.com/article/10.1023/A:1012400518207?error=cookies_not_supported&code=b74611f1-a6e5-471f-9e24-c02e186de39a 10.1023/A:1012400518207
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced showing a high similarity to bsh gene previously published. The resulting nucleotide sequence encodes a predicted protein of 317 amino acids, Mw = 35 kDa.
format Article
author Shuhaimi, M.
Ali, A.M.
Saleh, N.M.
Yazid, A.M.
spellingShingle Shuhaimi, M.
Ali, A.M.
Saleh, N.M.
Yazid, A.M.
Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
author_facet Shuhaimi, M.
Ali, A.M.
Saleh, N.M.
Yazid, A.M.
author_sort Shuhaimi, M.
title Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
title_short Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
title_full Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
title_fullStr Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
title_full_unstemmed Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum
title_sort cloning and sequence analysis of bile salt hydrolase (bsh) gene from bifidobacterium longum
publisher Springer Science and Business Media LLC
publishDate 2001
url http://psasir.upm.edu.my/id/eprint/115211/1/115211.pdf
http://psasir.upm.edu.my/id/eprint/115211/
https://link.springer.com/article/10.1023/A:1012400518207?error=cookies_not_supported&code=b74611f1-a6e5-471f-9e24-c02e186de39a
_version_ 1825162450586042368
score 13.244413

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