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Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins

Protein, being a fundamental biological macromolecule, assumes a critical function in various vital activities of cellular growth, heredity, reproduction, and other biological processes. Moreover, the utilization of recombinant proteins is extensive in the advancement of pharmaceutical materials, en...

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Main Authors: Eskandari, Azadeh, Thean, Chor Leow, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya
Format: Article
Published: Springer 2024
Online Access:http://psasir.upm.edu.my/id/eprint/115188/
https://link.springer.com/article/10.1007/s42485-024-00139-7?error=cookies_not_supported&code=de5be1b8-827e-4851-8c97-3da445d0327a
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spelling my.upm.eprints.1151882025-02-26T02:37:22Z http://psasir.upm.edu.my/id/eprint/115188/ Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins Eskandari, Azadeh Thean, Chor Leow Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya Protein, being a fundamental biological macromolecule, assumes a critical function in various vital activities of cellular growth, heredity, reproduction, and other biological processes. Moreover, the utilization of recombinant proteins is extensive in the advancement of pharmaceutical materials, enzyme industrial implementation, and fundamental proteomics investigation. Effective purification methods are necessary for the production of recombinant proteins as well as natural proteins and peptides to ensure their efficient production. Various methods have been developed to improve protein purification, incorporating the recent advancements in protein separation technology. This review is focused on the sophisticated approach to the purification techniques of protein, peptide, and recombinant protein. The system known as the multi-column plate adapter (MCPA) proves to be highly cost-effective in the field of protein purification methods. The assessment has determined that the method of employing an aqueous two-phase system (ATPS) is an economically sound, and highly efficient approach in protein purification. Thus, the ATPS might represent a feasible approach to single-stage purification, deviating from the complex chromatography procedure. The chromatography strategies have been improved for the purification of peptides including batch liquid, continuous and semi-continuous chromatography. In the realm of recombinant protein purification, affinity purification presents several advantages compared to alternative methods. To produce, identify, and purify recombinant proteins from their host systems in large quantities with high efficiency, affinity strategies—particularly fusion strategies—have been developed. Hence, the purpose of this review is to implement the various purification technologies, their potential employments, fundamentals, advantages, and limitations. Springer 2024-04-11 Article PeerReviewed Eskandari, Azadeh and Thean, Chor Leow and Abdul Rahman, Mohd Basyaruddin and Oslan, Siti Nurbaya (2024) Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins. Journal of Proteins and Proteomics, 15. pp. 233-257. ISSN 2524-4663 https://link.springer.com/article/10.1007/s42485-024-00139-7?error=cookies_not_supported&code=de5be1b8-827e-4851-8c97-3da445d0327a 10.1007/s42485-024-00139-7
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Protein, being a fundamental biological macromolecule, assumes a critical function in various vital activities of cellular growth, heredity, reproduction, and other biological processes. Moreover, the utilization of recombinant proteins is extensive in the advancement of pharmaceutical materials, enzyme industrial implementation, and fundamental proteomics investigation. Effective purification methods are necessary for the production of recombinant proteins as well as natural proteins and peptides to ensure their efficient production. Various methods have been developed to improve protein purification, incorporating the recent advancements in protein separation technology. This review is focused on the sophisticated approach to the purification techniques of protein, peptide, and recombinant protein. The system known as the multi-column plate adapter (MCPA) proves to be highly cost-effective in the field of protein purification methods. The assessment has determined that the method of employing an aqueous two-phase system (ATPS) is an economically sound, and highly efficient approach in protein purification. Thus, the ATPS might represent a feasible approach to single-stage purification, deviating from the complex chromatography procedure. The chromatography strategies have been improved for the purification of peptides including batch liquid, continuous and semi-continuous chromatography. In the realm of recombinant protein purification, affinity purification presents several advantages compared to alternative methods. To produce, identify, and purify recombinant proteins from their host systems in large quantities with high efficiency, affinity strategies—particularly fusion strategies—have been developed. Hence, the purpose of this review is to implement the various purification technologies, their potential employments, fundamentals, advantages, and limitations.
format Article
author Eskandari, Azadeh
Thean, Chor Leow
Abdul Rahman, Mohd Basyaruddin
Oslan, Siti Nurbaya
spellingShingle Eskandari, Azadeh
Thean, Chor Leow
Abdul Rahman, Mohd Basyaruddin
Oslan, Siti Nurbaya
Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
author_facet Eskandari, Azadeh
Thean, Chor Leow
Abdul Rahman, Mohd Basyaruddin
Oslan, Siti Nurbaya
author_sort Eskandari, Azadeh
title Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
title_short Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
title_full Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
title_fullStr Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
title_full_unstemmed Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
title_sort utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins
publisher Springer
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/115188/
https://link.springer.com/article/10.1007/s42485-024-00139-7?error=cookies_not_supported&code=de5be1b8-827e-4851-8c97-3da445d0327a
_version_ 1825162447485403136
score 13.244413

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