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Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate

Lipase from Candida rugosa was immobilized by entrapment on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) [poly(VP-co-HEMA)] hydrogel, cross-linked with ethylene glycol dimethacrylate (EDMA). The immobilized enzyme was used in the esterification of oleic acid with butanol in hexane. The...

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Main Authors: Basri, M., Wong, C. C., Ahmad, M. B., Razak, C. N. A., Salleh, A. B.
Format: Article
Language:English
Published: Wiley 1999
Online Access:http://psasir.upm.edu.my/id/eprint/115112/1/115112.pdf
http://psasir.upm.edu.my/id/eprint/115112/
https://aocs.onlinelibrary.wiley.com/doi/10.1007/s11746-999-0006-6
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spelling my.upm.eprints.1151122025-02-20T07:10:34Z http://psasir.upm.edu.my/id/eprint/115112/ Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate Basri, M. Wong, C. C. Ahmad, M. B. Razak, C. N. A. Salleh, A. B. Lipase from Candida rugosa was immobilized by entrapment on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) [poly(VP-co-HEMA)] hydrogel, cross-linked with ethylene glycol dimethacrylate (EDMA). The immobilized enzyme was used in the esterification of oleic acid with butanol in hexane. The activities of the immobilized enzyme preparations and the leaching of the enzyme from the hydrogel supports with respect to composition were investigated. The thermal, solvent, and storage stability of the immobilized preparations also were determined. Increasing the percentage VP from 0 to 90, which corresponds to the increase in the hydrophilicity of the hydrogels, increased the activity of the immobilized enzyme. Lipase immobilized onto VP(%):HEMA(%), 90:10 hydrogel had the highest activity. Increasing the hydrophobicity of the hydrogel (increasing the percentage HEMA) seemed to decrease leaching of the enzyme from the support. Immobilized lipase on 100% HEMA hydrogel indicated highest entrapment and lowest leaching by hexane washing. The lipase immobilized on VP(%):HEMA(%), 50:50 hydrogel showed highest thermal, solvent, and storage stability compared to lipase immobilized on other hydrogel compositions as well as the native lipase. Wiley 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115112/1/115112.pdf Basri, M. and Wong, C. C. and Ahmad, M. B. and Razak, C. N. A. and Salleh, A. B. (1999) Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate. Journal of the American Oil Chemists' Society, 76 (5). pp. 571-577. ISSN 0003-021X; eISSN: 1558-9331 https://aocs.onlinelibrary.wiley.com/doi/10.1007/s11746-999-0006-6 10.1007/s11746-999-0006-6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Lipase from Candida rugosa was immobilized by entrapment on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) [poly(VP-co-HEMA)] hydrogel, cross-linked with ethylene glycol dimethacrylate (EDMA). The immobilized enzyme was used in the esterification of oleic acid with butanol in hexane. The activities of the immobilized enzyme preparations and the leaching of the enzyme from the hydrogel supports with respect to composition were investigated. The thermal, solvent, and storage stability of the immobilized preparations also were determined. Increasing the percentage VP from 0 to 90, which corresponds to the increase in the hydrophilicity of the hydrogels, increased the activity of the immobilized enzyme. Lipase immobilized onto VP(%):HEMA(%), 90:10 hydrogel had the highest activity. Increasing the hydrophobicity of the hydrogel (increasing the percentage HEMA) seemed to decrease leaching of the enzyme from the support. Immobilized lipase on 100% HEMA hydrogel indicated highest entrapment and lowest leaching by hexane washing. The lipase immobilized on VP(%):HEMA(%), 50:50 hydrogel showed highest thermal, solvent, and storage stability compared to lipase immobilized on other hydrogel compositions as well as the native lipase.
format Article
author Basri, M.
Wong, C. C.
Ahmad, M. B.
Razak, C. N. A.
Salleh, A. B.
spellingShingle Basri, M.
Wong, C. C.
Ahmad, M. B.
Razak, C. N. A.
Salleh, A. B.
Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
author_facet Basri, M.
Wong, C. C.
Ahmad, M. B.
Razak, C. N. A.
Salleh, A. B.
author_sort Basri, M.
title Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
title_short Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
title_full Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
title_fullStr Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
title_full_unstemmed Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
title_sort immobilization of lipase on poly(n-vinyl-2-pyrrolidone-co-2-hydroxyethyl methacrylate) hydrogel for the synthesis of butyl oleate
publisher Wiley
publishDate 1999
url http://psasir.upm.edu.my/id/eprint/115112/1/115112.pdf
http://psasir.upm.edu.my/id/eprint/115112/
https://aocs.onlinelibrary.wiley.com/doi/10.1007/s11746-999-0006-6
_version_ 1825162434980085760
score 13.239859

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