Cover Image

Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation

Nylon tube was directly activated by alkaline glutaraldehyde solution. PEI was utilised as a spacer molecule. Glucose oxidase was immobilised to the nylon tube after reactivating the spacer molecules with glutaraldehyde. On immobilising glucose oxidase there was more protein binding and higher immob...

Full description

Saved in:
Bibliographic Details
Main Author: Salleh, Abu Bakar
Format: Article
Language:English
Published: Springer Science and Business Media LLC 1982
Online Access:http://psasir.upm.edu.my/id/eprint/115073/1/115073.pdf
http://psasir.upm.edu.my/id/eprint/115073/
https://link.springer.com/article/10.1007/BF00131150?error=cookies_not_supported&code=c1b6b9e1-173a-4106-8e9a-b87b964749ea
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.115073
record_format eprints
spelling my.upm.eprints.1150732025-02-19T07:25:30Z http://psasir.upm.edu.my/id/eprint/115073/ Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation Salleh, Abu Bakar Nylon tube was directly activated by alkaline glutaraldehyde solution. PEI was utilised as a spacer molecule. Glucose oxidase was immobilised to the nylon tube after reactivating the spacer molecules with glutaraldehyde. On immobilising glucose oxidase there was more protein binding and higher immobilised enzyme activity when compared to immobilised enzyme tube activated by triethyloxonium salt. The optimal condition for direct glutaraldehyde activation of nylon was incubation with 18.5% (w/v) glutaraldehyde in 0.12M borate pH 9.0 for 15 min at 90 °. Springer Science and Business Media LLC 1982 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115073/1/115073.pdf Salleh, Abu Bakar (1982) Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation. Biotechnology Letters, 4 (12). pp. 769-774. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1007/BF00131150?error=cookies_not_supported&code=c1b6b9e1-173a-4106-8e9a-b87b964749ea 10.1007/BF00131150
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Nylon tube was directly activated by alkaline glutaraldehyde solution. PEI was utilised as a spacer molecule. Glucose oxidase was immobilised to the nylon tube after reactivating the spacer molecules with glutaraldehyde. On immobilising glucose oxidase there was more protein binding and higher immobilised enzyme activity when compared to immobilised enzyme tube activated by triethyloxonium salt. The optimal condition for direct glutaraldehyde activation of nylon was incubation with 18.5% (w/v) glutaraldehyde in 0.12M borate pH 9.0 for 15 min at 90 °.
format Article
author Salleh, Abu Bakar
spellingShingle Salleh, Abu Bakar
Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
author_facet Salleh, Abu Bakar
author_sort Salleh, Abu Bakar
title Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
title_short Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
title_full Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
title_fullStr Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
title_full_unstemmed Activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
title_sort activation of nylon by alkaline glutaraldehyde solution for enzyme immobilisation
publisher Springer Science and Business Media LLC
publishDate 1982
url http://psasir.upm.edu.my/id/eprint/115073/1/115073.pdf
http://psasir.upm.edu.my/id/eprint/115073/
https://link.springer.com/article/10.1007/BF00131150?error=cookies_not_supported&code=c1b6b9e1-173a-4106-8e9a-b87b964749ea
_version_ 1825162427810971648
score 13.244413

Similar Items