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Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells

Many pathogens have developed strategies to avoid the host's immune system and hence improve their long-term survival. These strategies include antigenic variation, mimicry of host regulatory proteins and production of immunoregulatory molecules. The ruminant gastrointestinal nematode Trichostr...

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Main Authors: Riffkin, M.C., Seow, H.-F., Wood, P.R., Brown, L.E.
Format: Article
Language:English
Published: Wiley 2000
Online Access:http://psasir.upm.edu.my/id/eprint/115009/1/115009.pdf
http://psasir.upm.edu.my/id/eprint/115009/
https://onlinelibrary.wiley.com/doi/10.1046/j.1440-1711.2000.00948.x
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spelling my.upm.eprints.1150092025-02-14T06:58:39Z http://psasir.upm.edu.my/id/eprint/115009/ Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells Riffkin, M.C. Seow, H.-F. Wood, P.R. Brown, L.E. Many pathogens have developed strategies to avoid the host's immune system and hence improve their long-term survival. These strategies include antigenic variation, mimicry of host regulatory proteins and production of immunoregulatory molecules. The ruminant gastrointestinal nematode Trichostrongylus colubriformis produces several factors with homology to human immunoregulatory proteins. However, direct immunomodulation by T. colubriformis proteins has not yet been unequivocally demonstrated. Results in the present paper demonstrate that soluble T. colubriformis factors promote proliferation of the TNF-susceptible mouse fibrosarcoma cell line L929, while inhibiting proliferation of all other cell types tested. In addition, T. colubriformis homogenate enhanced the susceptibility of L929 cells to the cytotoxic action of ovine TNF-α. Within 1 h of exposure, T. colubriformis factors bind L929 cells in a stable fashion, yet it takes up to 24 h for the cells to become sensitised to TNF-α. Interestingly, the increase of both TNF-α sensitivity and proliferation of treated L929 cells correlated with an upregulation in expression of TNF-α p55 and p75 receptors. Wiley 2000-12-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115009/1/115009.pdf Riffkin, M.C. and Seow, H.-F. and Wood, P.R. and Brown, L.E. (2000) Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells. Immunology and Cell Biology, 78 (6). pp. 575-579. ISSN 0818-9641; eISSN: 0818-9641 https://onlinelibrary.wiley.com/doi/10.1046/j.1440-1711.2000.00948.x 10.1046/j.1440-1711.2000.00948.x
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Many pathogens have developed strategies to avoid the host's immune system and hence improve their long-term survival. These strategies include antigenic variation, mimicry of host regulatory proteins and production of immunoregulatory molecules. The ruminant gastrointestinal nematode Trichostrongylus colubriformis produces several factors with homology to human immunoregulatory proteins. However, direct immunomodulation by T. colubriformis proteins has not yet been unequivocally demonstrated. Results in the present paper demonstrate that soluble T. colubriformis factors promote proliferation of the TNF-susceptible mouse fibrosarcoma cell line L929, while inhibiting proliferation of all other cell types tested. In addition, T. colubriformis homogenate enhanced the susceptibility of L929 cells to the cytotoxic action of ovine TNF-α. Within 1 h of exposure, T. colubriformis factors bind L929 cells in a stable fashion, yet it takes up to 24 h for the cells to become sensitised to TNF-α. Interestingly, the increase of both TNF-α sensitivity and proliferation of treated L929 cells correlated with an upregulation in expression of TNF-α p55 and p75 receptors.
format Article
author Riffkin, M.C.
Seow, H.-F.
Wood, P.R.
Brown, L.E.
spellingShingle Riffkin, M.C.
Seow, H.-F.
Wood, P.R.
Brown, L.E.
Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells
author_facet Riffkin, M.C.
Seow, H.-F.
Wood, P.R.
Brown, L.E.
author_sort Riffkin, M.C.
title Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells
title_short Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells
title_full Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells
title_fullStr Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells
title_full_unstemmed Trichostrongylus colubriformis extract upregulates TNF-α receptor expression and enhances TNF-α sensitivity of L929 cells
title_sort trichostrongylus colubriformis extract upregulates tnf-α receptor expression and enhances tnf-α sensitivity of l929 cells
publisher Wiley
publishDate 2000
url http://psasir.upm.edu.my/id/eprint/115009/1/115009.pdf
http://psasir.upm.edu.my/id/eprint/115009/
https://onlinelibrary.wiley.com/doi/10.1046/j.1440-1711.2000.00948.x
_version_ 1825162414155366400
score 13.239859

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